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Disposable electrochemical immunosensor for analysis of cystatin C, a CKD biomarker

2019-08-15Journal article Open access
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dc.contributor.authorLOPES, Paula
dc.contributor.authorCosta Rama, Estefanía
dc.contributor.authorBeirão, Idalina
dc.contributor.authorNouws, Henri
dc.contributor.authorSantos-Silva, Alice
dc.contributor.authorDelerue-Matos, Cristina
dc.date.accessioned2020-06-25T14:27:36Z
dc.date.available2020-06-25T14:27:36Z
dc.date.issued2019-08-15
dc.description.abstractSpecific monitoring of cystatin C (CysC) levels in biological fluids is critical for diagnosis, treatment and mechanistic understanding of a spectrum of diseases, particularly chronic kidney disease (CKD). Despite evidences that CysC correlates with the high risk and/or progression of CKD, its use in clinical practice is still scarce. In this context, we report the development of a simple and sensitive immunosensor for the detection of CysC. The biosensor combines the technology of cost-effective screen-printed electrodes with the high specificity of a sandwich immunoassay. Optimized conditions showed that the sensor operates in a linear range between 10 and 100 ng mL-1, with a detection limit and a sensitivity of 6.0 ng mL-1 and 6.4 ± 0.3 μA ng mL-1 cm-2, respectively. Moreover, the sensor provided precise results (RSD ≤ 6.2%) and the quantification of CysC in CKD serum samples revealed to be in agreement with the values obtained by a particle-enhanced nephelometric immunoassay. In this light, the proposed immunosensor qualifies for clinical application, constituting a step forward in the development of fast, sensitive and cost-effective diagnostic tools that can improve the current medical care settings of CKD patients.pt_PT
dc.description.sponsorshipThis work was supported by European Union through projects Norte-01-0145-FEDER-000024 and Norte-01-0145-FEDER-000011, co-funded by FEDER in the scope of CCDR-N and NORTE2020 Partnership Agreement. This work also received financial support from the European Union (FEDER funds through COMPETE) and National Funds (FCT, Fundação para a Ciência e a Tecnologia) through project UID/QUI/50006/2013.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.talanta.2019.04.006pt_PT
dc.identifier.issn0039-9140
dc.identifier.urihttp://hdl.handle.net/10400.22/16012
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/abs/pii/S0039914019303959?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectElectrochemical immunosensorpt_PT
dc.subjectBiomarkerpt_PT
dc.subjectCystatin Cpt_PT
dc.subjectChronic kidney diseasept_PT
dc.subjectScreen-printed electrodespt_PT
dc.subjectGold nanoparticlespt_PT
dc.titleDisposable electrochemical immunosensor for analysis of cystatin C, a CKD biomarkerpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT
oaire.citation.endPage216pt_PT
oaire.citation.startPage211pt_PT
oaire.citation.titleTalantapt_PT
oaire.citation.volume201pt_PT
oaire.fundingStream5876
person.familyNameLOPES
person.familyNameCosta Rama
person.familyNameNouws
person.familyNameDelerue-Matos
person.givenNamePaula
person.givenNameEstefanía
person.givenNameHendrikus
person.givenNameCristina
person.identifier1046746
person.identifier.ciencia-id7C18-36BB-82B6
person.identifier.ciencia-id3614-E403-0136
person.identifier.ciencia-id9B1A-9CED-6EE3
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0003-4034-7722
person.identifier.orcid0000-0002-5357-9269
person.identifier.orcid0000-0002-1472-8866
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridJ-5815-2018
person.identifier.ridD-5249-2013
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id55808813100
person.identifier.scopus-author-id36703129800
person.identifier.scopus-author-id7801450247
person.identifier.scopus-author-id6603741848
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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