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Fast monolith-based chromatographic method for determination of methotrexate in drug delivery studies

2019Journal article Restricted access
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dc.contributor.authorBarbosa, Ana Isabel
dc.contributor.authorFernandes, Sara
dc.contributor.authorMachado, Sandia
dc.contributor.authorSousa, Patrícia
dc.contributor.authorSze, Ong Yong
dc.contributor.authorSilva, Eduarda M.P.
dc.contributor.authorBarreiros, Luisa
dc.contributor.authorLima, Sofia A.C.
dc.contributor.authorReis, Salette
dc.contributor.authorSegundo, Marcela A.
dc.date.accessioned2020-09-14T13:11:59Z
dc.date.available2020-09-14T13:11:59Z
dc.date.issued2019
dc.description.abstractMethotrexate (MTX) is a derivative of aminopterin, used as an anticancer or an anti-inflammatory agent. The development of suitable drug delivery systems containing MTX is an active area of research, requiring suitable analytical methods. Therefore, a high-throughput HPLC method is proposed for determination of MTX in the delivery system and permeation studies. Chromatographic separation was achieved on a reversed phase monolithic C18 column using isocratic elution (phosphate buffer (pH 7.0, 10 mM)-ACN (91:9, v/v)) and spectrophotometric detection at 302 nm. Total run time was 3.5 min, with MTX retention time of 2.1 min, providing 17 determinations per hour. The method was found to be specific, accurate (99.2–110%) and precise for intra-day (RSD ≤ 3.5%) and inter-day assays (RSD ≤ 3.4%). MTX showed stability after 24 h at room temperature or in the autosampler (4 °C) and over three freeze-thaw cycles with recoveries ≥94.2%. The validated method was successfully applied to establish in vitro drug release profile of MTX delivered by lipid nanoparticles. Application to pig skin permeation media provided mean recovery values ranging from 94.1 to 101.6% (RSD ≤ 1.1%).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.microc.2019.04.075pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/16264
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationPOCI/01/0145/FEDER/007265pt_PT
dc.relationNORTE-01-0145-FEDER-000011pt_PT
dc.relationAUTOMATION OF BIOCHEMICAL ASSAYS USING LAB-ON-VALVE AND BEAD INJECTION TECHNIQUES
dc.relationTargeting p53 family proteins: on the route to new anticancer agents
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0026265X19305934?via%3Dihubpt_PT
dc.subjectBioavailabilitypt_PT
dc.subjectMonolithic columnpt_PT
dc.subjectNanoparticlespt_PT
dc.subjectPermeationpt_PT
dc.titleFast monolith-based chromatographic method for determination of methotrexate in drug delivery studiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAUTOMATION OF BIOCHEMICAL ASSAYS USING LAB-ON-VALVE AND BEAD INJECTION TECHNIQUES
oaire.awardTitleTargeting p53 family proteins: on the route to new anticancer agents
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F89668%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FDTP-FTO%2F1981%2F2014/PT
oaire.citation.endPage189pt_PT
oaire.citation.startPage185pt_PT
oaire.citation.titleMicrochemical Journalpt_PT
oaire.citation.volume148pt_PT
oaire.fundingStream5876
oaire.fundingStream9471 - RIDTI
person.familyNameFernandes
person.familyNameBarreiros
person.givenNameSara
person.givenNameLuisa
person.identifier.ciencia-id1C12-D800-38A4
person.identifier.ciencia-id611F-E0C5-0230
person.identifier.orcid0000-0001-7042-1941
person.identifier.orcid0000-0003-3481-5809
person.identifier.ridD-7950-2013
person.identifier.scopus-author-id57203278917
person.identifier.scopus-author-id6508205485
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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