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Deciphering neuroimmune interactions in alcohol intake in mouse model of intermittent access in male and female

2024-05Conference poster Open access
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dc.contributor.authorPacheco, Raquel
dc.contributor.authorCanedo, Teresa
dc.contributor.authorRodrigues, Ana M.
dc.contributor.authorMoreira, Joana
dc.contributor.authorRelvas, João B.
dc.contributor.authorSocodato, Renato
dc.contributor.authorSummavielle, Teresa
dc.contributor.authorSummavielle, Teresa
dc.date.accessioned2025-05-15T14:41:18Z
dc.date.available2025-05-15T14:41:18Z
dc.date.issued2024-05
dc.description.abstractExcessive alcohol consumption continues to pose a significant global health challenge, with detrimental effects on millions of individuals. Our laboratory has shown that alcohol exposure triggers reactive changes in astrocytes, including alterations in gene expression, activity, and proliferation, while also affecting microglial morphology and immune responses. We are to characterizing the effects of chronic alcohol consumption using a wellestablished voluntary alcohol drinking model in adult mice, to investigate the impact of chronic alcohol exposure on the prefrontal cortex (PFC), focusing on glial cell morphology, synaptic density, and behaviour. Mice are exposed to intermittent “every-other-day” (EOD) access to alcohol 15% (v/v) for 3 weeks, and behaviourally tested for anxiety, depression and memory, before sacrifice at 21 days of alcohol, or at 7 days of withdrawal. Brains were processed for glial cell analysis. Our preliminary findings revealed sex-specific responses following chronic alcohol exposure. Male mice exhibited increased astrocyte volume in the ventromedial PFC (vmPFC) and hyper-ramification in the ventrolateral PFC (vlPFC), whereas females displayed reductions in astrocyte size and complexity. Microglia morphology also differed between sexes, with females showing increased cell volume and males displaying reduced microglial volume in the vlPFC. These results suggest distinctive immune and synaptic responses to ethanol in males and females. Of note, we observed heightened inhibitory synapse density in the male PFC, while females exhibited increased excitatory synapse density. We are now conducting a proteomic analysis of PFC synaptosomes to identify important molecular targets in the crosstalk between neuros and glial cells. With this work we expect to clarify the complex interplay between chronic ethanol exposure, sex, and PFC function, find also new targets for innovative therapeutic approaches.por
dc.identifier.citationPacheco, R., Canedo, T., Rodrigues, A. M., Moreira, J., Relvas, J. B., Socodato, R., & Summavielle, T. (2024). Deciphering neuroimmune interactions in alcohol intake in mouse model of intermittent access in male and female. Livro de Resumos do 17º Encontro de Investigação Jovem da U.Porto / Book of Abstracts Young Researchers Meeting of U.Porto, 1033–1034. https://www.up.pt/ijup/wp-content/uploads/sites/892/2024/05/Livro-de-Resumos_final.pdf
dc.identifier.isbn978-989-746-378-5
dc.identifier.urihttp://hdl.handle.net/10400.22/30088
dc.language.isoeng
dc.peerreviewedn/a
dc.publisherUniversidade do Porto
dc.relationPTDC/SAU-TOX/0067/2021
dc.relation.hasversionhttps://www.up.pt/ijup/wp-content/uploads/sites/892/2024/05/Livro-de-Resumos_final.pdf
dc.rights.uriN/A
dc.subjectAlcohol use
dc.subjectNeuroinflammation
dc.subjectSex differences
dc.titleDeciphering neuroimmune interactions in alcohol intake in mouse model of intermittent access in male and femalepor
dc.typeconference poster
dspace.entity.typePublication
oaire.citation.conferenceDate2024-05
oaire.citation.conferencePlacePorto
oaire.citation.endPage1034
oaire.citation.startPage1033
oaire.citation.titleLivro de Resumos do 17.º Encontro de Investigação Jovem da U.Porto / Book of Abstracts Young Researchers Meeting of U.Porto
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameSummavielle
person.givenNameTeresa
person.identifier677706
person.identifier.ciencia-idC41E-0816-5C85
person.identifier.orcid0000-0003-2548-6281
person.identifier.ridC-9776-2012
person.identifier.scopus-author-id6603092949
relation.isAuthorOfPublication207ee2de-85a0-4144-9e7e-b376c600e065
relation.isAuthorOfPublication.latestForDiscovery207ee2de-85a0-4144-9e7e-b376c600e065

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