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Advisor(s)
Abstract(s)
Prostate Specific Antigen (PSA) is widely used as a biomarker for prostate cancer. Recently, an electrochemical biosensor for PSA detection by means ofmolecularly imprinted polymers (MIPs)was developed. Thiswork evaluated the performance and the effectiveness of that PSA biosensor in screening the biomarker PSA in biological mediawith complex composition, collected fromdifferent human prostate cell line cultures. For that, the prostate cancer LNCaP and PC3 cells, and the non-cancerous prostate cell line PNT2 were cultured for 2, 7 and 14 days in eitherα-MEMor RPMI in the presence of 10% or 30% fetal bovine serum. Human gingival fibroblastswere used as a non-cancerous non-prostatic control. The different culture conditions modulated cellular proliferation and the expression of several prostate markers, including PSA. The electrochemical biosensor was able to specifically detect PSA in the culture media and values obtained were similar to those achieved by a commercial Enzyme- Linked Immunosorbent Assay (ELISA) kit, the most commonly used method for PSA quantification in prostate cancer diagnosis. Thus, the tested biosensor may represent a useful alternative as a diagnostic tool for PSA determination in biological samples.
Description
Keywords
Prostate Specific Antigen Electrochemical biosensor Prostate cell lines
Citation
Rebelo, T. S. C. R., Noronha, J. P., Galésio, M., Santos, H., Diniz, M., Sales, M. G. F., Fernandes, M. H., & Costa-Rodrigues, J. (2016). Testing the variability of PSA expression by different human prostate cancer cell lines by means of a new potentiometric device employing molecularly antibody assembled on graphene surface. Materials Science and Engineering: C, 59, 1069–1078. https://doi.org/10.1016/j.msec.2015.11.032
Publisher
Elsevier