Publication
Backside-surface imprinting as a new strategy to generate specific plastic antibody materials
| dc.contributor.author | Cabral-Miranda, Gustavo | |
| dc.contributor.author | Sales, M. Goreti F. | |
| dc.contributor.author | Gidlund, Magnus | |
| dc.date.accessioned | 2015-10-16T12:09:43Z | |
| dc.date.available | 2015-10-16T12:09:43Z | |
| dc.date.issued | 2014 | |
| dc.description.abstract | A backside protein-surface imprinting process is presented herein as a novel way to generate specific synthetic antibody materials. The template is covalently bonded to a carboxylated-PVC supporting film previously cast on gold, let to interact with charged monomers and surrounded next by another thick polymer. This polymer is then covalently attached to a transducing element and the backside of this structure (supporting film plus template) is removed as a regular “tape”. The new sensing layer is exposed after the full template removal, showing a high density of re-binding positions, as evidenced by SEM. To ensure that the templates have been efficiently removed, this re-binding layer was cleaned further with a proteolytic enzyme and solution washout. The final material was named MAPS, as in the back-side reading of SPAM, because it acts as a back-side imprinting of this recent approach. It was able to generate, for the first time, a specific response to a complex biomolecule from a synthetic material. Non-imprinted materials (NIMs) were also produced as blank and were used as a control of the imprinting process. All chemical modifications were followed by electrochemical techniques. This was done on a supporting film and transducing element of both MAPS and NIM. Only the MAPS-based device responded to oxLDL and the sensing layer was insensitive to other serum proteins, such as myoglobin and haemoglobin. Linear behaviour between log(C, μg mL−1) versus charged tranfer resistance (RCT, Ω) was observed by electrochemical impedance spectroscopy (EIS). Calibrations made in Fetal Calf Serum (FCS) were linear from 2.5 to 12.5 μg mL−1 (RCT = 946.12 × log C + 1590.7) with an R-squared of 0.9966. Overall, these were promising results towards the design of materials acting close to the natural antibodies and applied to practical use of clinical interest. | pt_PT |
| dc.identifier.doi | 10.1039/C3TB21740J | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10400.22/6725 | |
| dc.language.iso | eng | pt_PT |
| dc.publisher | Royal Society of Chemistry | pt_PT |
| dc.relation.publisherversion | http://pubs.rsc.org/EN/content/articlelanding/2014/tb/c3tb21740j#!divAbstract | pt_PT |
| dc.title | Backside-surface imprinting as a new strategy to generate specific plastic antibody materials | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 3095 | pt_PT |
| oaire.citation.startPage | 3087 | pt_PT |
| oaire.citation.title | Journal of Materials Chemistry B | pt_PT |
| oaire.citation.volume | 2 | pt_PT |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |