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Assessing the antitumor potential of variants of the extracellular carbohydrate polymer from synechocystis ΔsigF mutant

2023-03-10Journal article Open access
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dc.contributor.authorMota, Rita
dc.contributor.authorLima, Raquel T.
dc.contributor.authorFlores, Carlos
dc.contributor.authorSilva, Juliana F.
dc.contributor.authorCruz, Beatriz
dc.contributor.authorAlves, Bárbara
dc.contributor.authorPinto, Marta T.
dc.contributor.authorAdessi, Alessandra
dc.contributor.authorPereira, Sara B.
dc.contributor.authorDe Philippis, Roberto
dc.contributor.authorSoares, Paula
dc.contributor.authorTamagnini, Paula
dc.date.accessioned2023-06-05T15:45:18Z
dc.date.available2023-06-05T15:45:18Z
dc.date.issued2023-03-10
dc.description.abstractCancer is a leading cause of death worldwide with a huge societal and economic impact. Clinically effective and less expensive anticancer agents derived from natural sources can help to overcome limitations and negative side effects of chemotherapy and radiotherapy. Previously, we showed that the extracellular carbohydrate polymer of a Synechocystis ΔsigF overproducing mutant displayed a strong antitumor activity towards several human tumor cell lines, by inducing high levels of apoptosis through p53 and caspase-3 activation. Here, the ΔsigF polymer was manipulated to obtain variants that were tested in a human melanoma (Mewo) cell line. Our results demonstrated that high molecular mass fractions were important for the polymer bioactivity, and that the reduction of the peptide content generated a variant with enhanced in vitro antitumor activity. This variant, and the original ΔsigF polymer, were further tested in vivo using the chick chorioallantoic membrane (CAM) assay. Both polymers significantly decreased xenografted CAM tumor growth and affected tumor morphology, by promoting less compact tumors, validating their antitumor potential in vivo. This work contributes with strategies for the design and testing tailored cyanobacterial extracellular polymers and further strengths the relevance of evaluating this type of polymers for biotechnological/biomedical applications.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMota, R., Lima, R. T., Flores, C., Silva, J. F., Cruz, B., Alves, B., Pinto, M. T., Adessi, A., Pereira, S. B., De Philippis, R., Soares, P., & Tamagnini, P. (2023). Assessing the Antitumor Potential of Variants of the Extracellular Carbohydrate Polymer from Synechocystis ΔsigF Mutant. Polymers, 15(6), 1382. https://doi.org/10.3390/polym15061382pt_PT
dc.identifier.doi10.3390/polym15061382pt_PT
dc.identifier.eissn2073-4360
dc.identifier.urihttp://hdl.handle.net/10400.22/23078
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2073-4360/15/6/1382pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAntitumor activitypt_PT
dc.subjectCAM assaypt_PT
dc.subjectCancerpt_PT
dc.subjectCarbohydrate polymerpt_PT
dc.subjectCyanobacteriapt_PT
dc.subjectSynechocystispt_PT
dc.titleAssessing the antitumor potential of variants of the extracellular carbohydrate polymer from synechocystis ΔsigF mutantpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage14pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titlePolymerspt_PT
oaire.citation.volume15(6)pt_PT
person.familyNameCouto Viana Alves
person.givenNameBárbara
person.identifier2824525
person.identifier.orcid0000-0003-1743-6022
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication9e287a17-721b-422f-a26b-b6e1437582c9
relation.isAuthorOfPublication.latestForDiscovery9e287a17-721b-422f-a26b-b6e1437582c9

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