Publication
Assess of acid tolerance of non-typhoidal Salmonella and Enterococcus faecium from different epidemiological and genetic backgrounds
| dc.contributor.author | Rebelo, Andreia | |
| dc.contributor.author | Mourão, Joana | |
| dc.contributor.author | Freitas, Ana R. | |
| dc.contributor.author | Campos, Joana | |
| dc.contributor.author | Peixe, Luísa | |
| dc.contributor.author | Antunes, Patrícia | |
| dc.contributor.author | Novais, Carla | |
| dc.date.accessioned | 2024-12-16T15:49:48Z | |
| dc.date.available | 2024-12-16T15:49:48Z | |
| dc.date.issued | 2019-12 | |
| dc.description.abstract | Acid stress is one of the most frequently encountered hostile conditions that bacteria have to face (e.g. foodproduction-chain/feed/disinfectants/human and animal hosts). Nevertheless, acid tolerance profile of bacteria from diverse epidemiological and genetic backgrounds, including multidrug- resistant (MDR), is still poorly explored. The aim of this study was to assess the susceptibility to acidic-pH of non-typhoidal Salmonella and Enterococcus faecium (Efm) from diverse origins. We included Salmonella (n=66; 23 serotypes) and Efm (n=74; clades A1/A2/B) recovered from human-n=54, food-animal production setting-n=20, food- n=56) and environment-n=10 (1997-2018; 6-countries). The minimum-growth-pH (growth-pHmin) was assessed by broth-microdilution using Mueller-Hinton-II adjusted with HCl (pH=2.0-6.5/16h-20h ±2h/37ºC) and the minimum-survival-pH (survival-pHmin) by plating the microdilution wells without visible growth in Brain-HeartInfusion-agar (BHI) (24h-48h±2h/37ºC). An Acid-Tolerance-Response (ATR) assay was performed in 3 isolates of each genera (different growth/survival-pHmin), exposing bacteria in log-phase to an acid-shockchallenge (pH=3.0/15’-Salmonella/60’-Efm) or to a pre- adaptation to acidic-pH (pH=4.5/60’ - for both bacteria) followed by an acid-shock-challenge (pH=3.0/15’-Salmonella/60’-Efm). After that a growth-pHmin and survival-pHmin assays were performed. Most Salmonella showed a growth-pHmin of 4.0 (98%- n=65/66) and a survival-pHmin between 4.0 (52%- n=34/66) and 3.5 (48%-n=32/66). In Efm, the growth-pHmin ranged between 4.5 (65%-n=48/74) and 5.0 (35%-n=26/74) and the survival-pHmin between 3.0 (15%-n=11/74), 3.5 (43%- n=32/74) and 4.0 (42%n=31/74). Only Efm isolates from food (39%-n=11/28), mostly from a poultry-processing-plant using peracetic acid as disinfectant (73%-n=8/11), presented the lowest survival-pHmin 3.0 (73%- MDR/82%-clade A2). Similar survival-pHmin=3.5-4.0 were observed for different Salmonella serotypes and Efm clades. However, a higher percentage of MDR-Salmonella (61%-n=27/44) were able to survive at pH=3.5 contrasting with nonMDR-Salmonella (23%-n=5/22) (p<0,05; Fisher-exact test). The ATR-assay (pre-adaptation acidic pH+acid shock challenge) enhanced survival-pHmin from 3 to 2.5 in 1-Efm (clade A2/MDR/from a poultry processing plant) and from 4 to 3.5 in 1-S. 4,[5],12:i:- (MDR/with mcr-1 gene/from pork meat). Our data suggest that MDR-Salmonella and Efm with diverse epidemiological and genetic backgrounds can survive to low-pH values, although differences among clades/serotypes were not detected. MDR- Salmonella showed a better ability to survive to more acidic pH than non-MDR isolates. ATR-assays revealed strainspecific ability to survive under more acidic-pH after a pre-adaptation to middle acidic- pH. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Rebelo, A., Mourão, J., Freitas, A. R., Campos, Joana, Peixe, Luísa, & Novais, C. (2019). Assess of acid tolerance of non-typhoidal Salmonella and Enterococcus faecium from different epidemiological and genetic backgrounds. Book of abstracts - Congress of Microbiology and Biotechnology 2019 - MICROBIOTEC 19, 457. | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10400.22/26875 | |
| dc.language.iso | eng | pt_PT |
| dc.publisher | Universidade de Coimbra/Sociedade Portuguesa de Microbiologia/Sociedade Portuguesa de Biotecnologia | pt_PT |
| dc.relation.publisherversion | https://microbiotec19.net/test/wp-content/uploads/2019/07/Book_abstract_online.pdf | pt_PT |
| dc.subject | Acid tolerance | pt_PT |
| dc.subject | Non-typhoidal Salmonella | pt_PT |
| dc.subject | Enterococcus faecium | pt_PT |
| dc.title | Assess of acid tolerance of non-typhoidal Salmonella and Enterococcus faecium from different epidemiological and genetic backgrounds | pt_PT |
| dc.type | conference object | |
| dspace.entity.type | Publication | |
| oaire.citation.conferencePlace | Coimbra | pt_PT |
| oaire.citation.startPage | 457 | pt_PT |
| oaire.citation.title | Book of abstracts - Congress of Microbiology and Biotechnology 2019 - MICROBIOTEC 19 | pt_PT |
| person.familyName | Rebelo | |
| person.givenName | Andreia | |
| person.identifier.ciencia-id | A81C-7A59-ECAD | |
| person.identifier.orcid | 0000-0003-2218-6005 | |
| person.identifier.rid | T-9024-2017 | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | conferenceObject | pt_PT |
| relation.isAuthorOfPublication | 913dc5cd-9e92-456d-88f5-419571a87f85 | |
| relation.isAuthorOfPublication.latestForDiscovery | 913dc5cd-9e92-456d-88f5-419571a87f85 |