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Advisor(s)
Abstract(s)
Colorectal carcinoma is a major health concern worldwide and its high incidence and mortality require
accurate screening methods. Following endoscopic examination, polyps must be removed for histopathological
characterization. Aiming to contribute to the improvement of current endoscopy methods of colorectal carcinoma
screening or even for future development of laser treatment procedures, we studied the diffusion properties of
glucose and water in colorectal healthy and pathological mucosa. These parameters characterize the tissue
dehydration and the refractive index matching mechanisms of optical clearing (OC). We used ex vivo tissues
to measure the collimated transmittance spectra and thickness during treatments with OC solutions containing
glucose in different concentrations. These time dependencies allowed for estimating the diffusion time and diffusion
coefficient values of glucose and water in both types of tissues. The measured diffusion times for glucose
in healthy and pathological mucosa samples were 299.2+/-4.7 s and 320.6+/-10.6 s for 40% and 35% glucose
concentrations, respectively. Such a difference indicates a slower glucose diffusion in cancer tissues, which
originate from their ability to trap far more glucose than healthy tissues. We have also found a higher free
water content in cancerous tissue that is estimated as 64.4% instead of 59.4% for healthy mucosa.
Description
Keywords
Colorectal tissue Cancer Free and bound water Optical clearing Diffusion coefficient Tissue dehydration
Citation
Glucose diffusion in colorectal mucosa: a comparative study between normal and cancer tissues," J. Biomed. Opt. 22(9), 091506 (2017), doi: 10.1117/1.JBO.22.9.091506
Publisher
Society of Photo-optical Instrumentation Engineers