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Turning a Collagenesis-Inducing Peptide Into a Potent Antibacterial and Antibiofilm Agent Against Multidrug-Resistant Gram-Negative Bacteria

2019Journal article Open access
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dc.contributor.authorGomes, Ana
dc.contributor.authorBessa, Lucinda J.
dc.contributor.authorFernandes, Iva
dc.contributor.authorFerraz, Ricardo
dc.contributor.authorMateus, Nuno
dc.contributor.authorGameiro, Paula
dc.contributor.authorTeixeira, Cátia
dc.contributor.authorGomes, Paula
dc.date.accessioned2019-09-18T14:46:49Z
dc.date.available2019-09-18T14:46:49Z
dc.date.issued2019
dc.description.abstractAntimicrobial resistance is becoming one the most serious health threats worldwide, as it not only hampers effective treatment of infectious diseases using current antibiotics, but also increases the risks of medical procedures like surgery, transplantation, bone and dental implantation, chemotherapy, or chronic wound management. To date, there are no effective measures to tackle life-threatening nosocomial infections caused by multidrug resistant bacterial species, of which Gram-negative species within the so-called "ESKAPE" pathogens are the most worrisome. Many such bacteria are frequently isolated from severely infected skin lesions such as diabetic foot ulcers (DFU). In this connection, we are pursuing new peptide constructs encompassing antimicrobial and collagenesis-inducing motifs, to tackle skin and soft tissue infections by exerting a dual effect: antimicrobial protection and faster healing of the wound. This produced peptide 3.1-PP4 showed MIC values as low as 1.0 and 2.1 μM against Escherichia coli and Pseudomonas aeruginosa, respectively, and low toxicity to HFF-1 human fibroblasts. Remarkably, the peptide was also potent against multidrug-resistant isolates of Klebsiella pneumoniae, E. coli, and P. aeruginosa (MIC values between 0.5 and 4.1 μM), and hampered the formation of/disaggregated K. pneumoniae biofilms of resistant clinical isolates. Moreover, this notable hybrid peptide retained the collagenesis-inducing behavior of the reference cosmeceutical peptide C16-PP4 ("Matrixyl"). In conclusion, 3.1-PP4 is a highly promising lead toward development of a topical treatment for severely infected skin injuries.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGomes, A., Bessa, L. J., Fernandes, I., Ferraz, R., Mateus, N., Gameiro, P., Teixeira, C., & Gomes, P. (2019). Turning a Collagenesis-Inducing Peptide Into a Potent Antibacterial and Antibiofilm Agent Against Multidrug-Resistant Gram-Negative Bacteria. Frontiers in Microbiology, 10. https://www.frontiersin.org/articles/10.3389/fmicb.2019.01915
dc.identifier.doi10.3389/fmicb.2019.01915
dc.identifier.urihttp://hdl.handle.net/10400.22/14645
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relation.publisherversionhttps://www.frontiersin.org/articles/10.3389/fmicb.2019.01915/fullpt_PT
dc.subjectantibiofilmpt_PT
dc.subjectwound-healingpt_PT
dc.subjectantimicrobial peptidept_PT
dc.subjectmultidrug-resistant bacteriapt_PT
dc.subjectcollagenpt_PT
dc.subjectKlebsiella pneumoniaept_PT
dc.subjectESKAPEpt_PT
dc.titleTurning a Collagenesis-Inducing Peptide Into a Potent Antibacterial and Antibiofilm Agent Against Multidrug-Resistant Gram-Negative Bacteriapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage1915pt_PT
oaire.citation.titleFrontiers in Microbiologypt_PT
oaire.citation.volume10pt_PT
person.familyNameFerraz
person.givenNameRicardo
person.identifier.ciencia-id001E-71CE-F92D
person.identifier.orcid0000-0002-1761-117X
person.identifier.ridG-5639-2011
person.identifier.scopus-author-id24464208500
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationa5a8faa7-12a5-4b1c-bced-44c895677397
relation.isAuthorOfPublication.latestForDiscoverya5a8faa7-12a5-4b1c-bced-44c895677397

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