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ESBL and AmpC β-Lactamases in Clinical Strains of Escherichia coli from Serra da Estrela, Portugal

2019Journal article Open access
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dc.contributor.authorOliveira, Cátia
dc.contributor.authorAmador, Paula
dc.contributor.authorPrudêncio, Cristina
dc.contributor.authorTomaz, Cândida T
dc.contributor.authorTavares-Ratado, Paulo
dc.contributor.authorFernandes, Rúben
dc.date.accessioned2019-11-21T14:47:48Z
dc.date.available2019-11-21T14:47:48Z
dc.date.issued2019
dc.description.abstractBackground and Objectives: Given the considerable spatial, temporal, and ecological factors, heterogeneity, which affects emergency response, persistence, and dissemination of genetic determinants that confer microorganisms their resistance to antibiotics, several authors claim that antibiotics' resistance must be perceived as an ecological problem. The aim of this study was to determine the prevalence of broad-spectrum bla genes, not only Extended-spectrum β-lactamases (ESBL) but also AmpC-types, in clinical strains of Escherichia coli isolated from Portugal (in the highest region of the country, Serra da Estrela) to disclose susceptibility profiles among different genotypes, and to compare the distribution of bla genes expressing broad-spectrum enzymes. Materials and Methods: Clinical strains of Escherichia coli presenting resistance to third generation (3G) cephalosporins and susceptibility to inhibition by clavulanic acid were studied by means of phenotypic and molecular profiling techniques for encoding β-lactamases genes. Results: Strains were mainly isolated from hospital populations (97%). Molecular analysis enabled the detection of 49 bla genes, in which 55% (27/49) were identified as blaOXA-1-like, 33% (16/49) as blaCTX-M-group-1, 10% (5/49) as blaTEM, and 2% (1/49) were identified as genes blaCIT (AmpC). Among all blaOXA-1-like detected, about 59% of strains expressed at least another bla gene. Co-production of β-lactamases was observed in 40% of strains, with the co-production of CTX-M group 1 and OXA-1-like occurring as the most frequent. Conclusions: This is the first study using microorganisms isolated from native people from the highest Portuguese mountain regions, showing an unprecedent high prevalence of genes blaOXA-1-like in this country.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationOliveira, C., Amador, P., Prudêncio, C., Tomaz, C. T., Tavares-Ratado, P., & Fernandes, R. (2019). ESBL and AmpC β-Lactamases in Clinical Strains of Escherichia coli from Serra da Estrela, Portugal. Medicina, 55(6), Artigo 6. https://doi.org/10.3390/medicina55060272
dc.identifier.doi10.3390/medicina55060272pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/14897
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relation.publisherversionhttps://www.mdpi.com/1648-9144/55/6/272pt_PT
dc.subjectβ-lactamasespt_PT
dc.subjectEscherichia colipt_PT
dc.subjectESBLpt_PT
dc.subjectResistance mechanismspt_PT
dc.subjectSerra da Estrelapt_PT
dc.titleESBL and AmpC β-Lactamases in Clinical Strains of Escherichia coli from Serra da Estrela, Portugalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue6pt_PT
oaire.citation.startPage272pt_PT
oaire.citation.titleMedicinapt_PT
oaire.citation.volume55pt_PT
person.familyNamePrudêncio
person.familyNameFernandes
person.givenNameCristina
person.givenNameRúben
person.identifier1200571
person.identifier635792
person.identifier.ciencia-idC81E-F4EE-FADE
person.identifier.ciencia-id0D1F-4090-E82A
person.identifier.orcid0000-0002-9920-936X
person.identifier.orcid0000-0001-8933-3984
person.identifier.scopus-author-id6508057930
person.identifier.scopus-author-id57640135700
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication881a8ad5-ab13-4e49-89f4-08ca61cc81e3
relation.isAuthorOfPublication9e6c397c-62d8-4a40-8ec7-ad05ae0ebcc4
relation.isAuthorOfPublication.latestForDiscovery9e6c397c-62d8-4a40-8ec7-ad05ae0ebcc4

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