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Breast cancer biomarker (HER2-ECD) detection using a molecularly imprinted electrochemical sensor

2018-06Journal article Open access
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dc.contributor.authorPacheco, João
dc.contributor.authorRebelo, Patrícia
dc.contributor.authorFreitas, Maria
dc.contributor.authorNouws, Henri
dc.contributor.authorDelerue-Matos, Cristina
dc.date.accessioned2019-09-12T13:27:50Z
dc.date.available2019-09-12T13:27:50Z
dc.date.issued2018-06
dc.description.abstractThe extracellular domain of the human epidermal growth factor receptor 2 (HER2-ECD) is a protein breast cancer biomarker. Its quantification in peripheral blood could provide an important contribution to diagnostics and patient follow-up. In this work an electrochemical molecularly imprinted polymer (MIP) sensor for the quantification of HER2-ECD was developed. The MIP was electropolymerized by cyclic voltammetry using a solution containing phenol and HER2-ECD on a screen-printed gold electrode (AuSPE). The sensor was characterized by cyclic voltammetry and electrochemical impedance spectroscopy. The analysis of HER2-ECD was performed by differential pulse voltammetry using ([Fe(CN)6]3−/4−as redox probe. The linear range was established in the concentration interval from 10 to 70 ng/mL HER2-ECD, with a limit of detection of 1.6 ng/L and a limit of quantification of 5.2 ng/mL. Through the analysis of other protein biomarkers, the MIP sensor was found to be selective. Furthermore, these proteins did not interfere in the analysis of the selected biomarker. The developed sensor was used for the analysis of spiked human serum samples, providing adequate recovery values and precise results. The outcomes of this study indicate that the developed MIP sensor could be useful in the non-invasive analysis of HER2-ECD in breast cancer patients.pt_PT
dc.description.sponsorshipMaria Freitas is grateful to FCT (Fundação para a Ciência e a Tecnologia) for her PhD grant (SFRH/BD/111942/2015). Patrícia Rebelo is grateful to FCT (Fundação para a Ciência e a Tecnologia) for her PhD grant (SFRH/BD/132384/2017). João Pacheco is grateful to FCT for his Postdoc. grant (SFRH/BPD/101419/2014), financed by POPH-QREN-Tipologia 4.1-Formação Avançada, subsidized by Fundo Social Europeu and Ministério da Ciência, Tecnologia e Ensino Superior. This work received financial support from the European Union (FEDER funds through COMPETE) and National Funds (FCT) through project UID/QUI/50006/2013.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.snb.2018.06.113
dc.identifier.issn0925-4005
dc.identifier.urihttp://hdl.handle.net/10400.22/14573
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0925400518312188?via%3Dihubpt_PT
dc.subjectBreast cancerpt_PT
dc.subjectMolecularly imprinted polymerpt_PT
dc.subjectHER2pt_PT
dc.subjectElectrochemical sensorpt_PT
dc.subjectVoltammetrypt_PT
dc.titleBreast cancer biomarker (HER2-ECD) detection using a molecularly imprinted electrochemical sensorpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT
oaire.citation.endPage1014pt_PT
oaire.citation.startPage1008pt_PT
oaire.citation.titleSensors and Actuators B: Chemicalpt_PT
oaire.citation.volume273pt_PT
oaire.fundingStream5876
person.familyNamePacheco
person.familyNameRebelo
person.familyNameNouws
person.familyNameDelerue-Matos
person.givenNameJoão
person.givenNamePatrícia
person.givenNameHendrikus
person.givenNameCristina
person.identifier1046746
person.identifier.ciencia-id8D13-F178-F44D
person.identifier.ciencia-id6112-1293-B25E
person.identifier.ciencia-idBC12-319B-037F
person.identifier.ciencia-id9B1A-9CED-6EE3
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0001-8366-0490
person.identifier.orcid0000-0001-5209-668X
person.identifier.orcid0000-0002-0628-2753
person.identifier.orcid0000-0002-1472-8866
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridD-5249-2013
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id35519962400
person.identifier.scopus-author-id7801450247
person.identifier.scopus-author-id6603741848
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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