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Detection of Arah1 (a major peanut allergen) in food using an electrochemical gold nanoparticle-coated screen-printed immunosensor

2015-02Journal article Open access
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dc.contributor.authorAlves, Rita C.
dc.contributor.authorPimentel, Filipa B.
dc.contributor.authorNouws, Henri P.A.
dc.contributor.authorMarques, Raquel C.B.
dc.contributor.authorGonzález-García, María Begoña
dc.contributor.authorOliveira, M. Beatriz P.P.
dc.contributor.authorDelerue-Matos, Cristina
dc.date.accessioned2015-12-23T16:06:55Z
dc.date.available2015-12-23T16:06:55Z
dc.date.issued2015-02
dc.description.abstractA gold nanoparticle-coated screen-printed carbon electrode was used as the transducer in the development of an electrochemical immunosensor for Ara h 1 (a major peanut allergen) detection in food samples. Gold nanoparticles (average diameter=32 nm) were electrochemically generated on the surface of screen-printed carbon electrodes. Two monoclonal antibodies were used in a sandwich-type immunoassay and the antibody–antigen interaction was electrochemically detected through stripping analysis of enzymatically (using alkaline phosphatase) deposited silver. The total time of the optimized immunoassay was 3 h 50 min. The developed immunosensor allowed the quantification of Ara h 1 between 12.6 and 2000 ng/ml, with a limit of detection of 3.8 ng/ml, and provided precise (RSD <8.7%) and accurate (recovery >96.6%) results. The immunosensor was successfully applied to the analysis of complex food matrices (cookies and chocolate), being able to detect Ara h 1 in samples containing 0.1% of peanut.pt_PT
dc.identifier.doi10.1016/j.bios.2014.08.026pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/7255
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relation.ispartofseriesBiosensors and Bioelectronics;Vol. 64
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0956566314006149pt_PT
dc.subjectElectrochemical immunosensorpt_PT
dc.subjectBiosensorpt_PT
dc.subjectScreen-printed carbon electrodept_PT
dc.subjectAllergenpt_PT
dc.subjectPeanutpt_PT
dc.subjectAra h 1pt_PT
dc.titleDetection of Arah1 (a major peanut allergen) in food using an electrochemical gold nanoparticle-coated screen-printed immunosensorpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage24pt_PT
oaire.citation.startPage19pt_PT
oaire.citation.titleBiosensors and Bioelectronicspt_PT
oaire.citation.volume64pt_PT
person.familyNameAlves
person.familyNameDelerue-Matos
person.givenNameRita C.
person.givenNameCristina
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0002-5053-513X
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridI-9081-2012
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id7102691286
person.identifier.scopus-author-id6603741848
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication4b5cf5a1-8445-4d6a-ac30-5fbee0326269
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication.latestForDiscovery09f6a7bd-2f15-42b0-adc5-04bd22210519

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