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Photodynamic inactivation of recombinant bioluminescent Escherichia coli by cationic porphyrins under artificial and solar irradiation

2008-11-01Journal article Open access
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dc.contributor.authorAlves, Eliana
dc.contributor.authorCarvalho, Carla M. B.
dc.contributor.authorTomé, João P. C.
dc.contributor.authorFaustino, Maria A. F.
dc.contributor.authorNeves, Maria G. P. M. S.
dc.contributor.authorTomé, Augusto C.
dc.contributor.authorCavaleiro, João A. S.
dc.contributor.authorCunha, Ângela
dc.contributor.authorMendo, Sónia
dc.contributor.authorAlmeida, Adelaide
dc.date.accessioned2021-09-28T07:33:15Z
dc.date.available2021-09-28T07:33:15Z
dc.date.issued2008-11-01
dc.description.abstractA faster and simpler method to monitor the photoinactivation process of Escherichia coli involving the use of recombinant bioluminescent bacteria is described here. Escherichia coli cells were transformed with luxCDABE genes from the marine bioluminescent bacterium Vibrio fischeri and the recombinant bioluminescent indicator strain was used to assess, in real time, the effect of three cationic meso-substituted porphyrin derivatives on their metabolic activity, under artificial (40 W m−2) and solar irradiation (≈620 W m−2). The photoinactivation of bioluminescent E. coli is effective (>4 log bioluminescence decrease) with the three porphyrins used, the tricationic porphyrin Tri-Py+-Me-PF being the most efficient compound. The photoinactivation process is efficient both with solar and artificial light, for the three porphyrins tested. The results show that bioluminescence analysis is an efficient and sensitive approach being, in addition, more affordable, faster, cheaper and much less laborious than conventional methods. This approach can be used as a screening method for bacterial photoinactivation studies in vitro and also for the monitoring of the efficiency of novel photosensitizer molecules. As far as we know, this is the first study involving the use of bioluminescent bacteria to monitor the antibacterial activity of porphyrins under environmental conditions.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAlves, E., Carvalho, C. M. B., Tomé, J. P. C., Faustino, M. A. F., Neves, M. G. P. M. S., Tomé, A. C., Cavaleiro, J. A. S., Cunha, Â., Mendo, S., & Almeida, A. (2008). Photodynamic inactivation of recombinant bioluminescent Escherichia coli by cationic porphyrins under artificial and solar irradiation. Journal of Industrial Microbiology and Biotechnology, 35(11), 1447-1447. 10.1007/s10295-008-0446-2pt_PT
dc.identifier.doi10.1007/s10295-008-0446-2pt_PT
dc.identifier.issn 1476-5535
dc.identifier.urihttp://hdl.handle.net/10400.22/18577
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherOxford Academicpt_PT
dc.relation.publisherversionhttps://academic.oup.com/jimb/article/35/11/1447/5993262pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCationic porphyrinspt_PT
dc.subjectPhotodynamic antimicrobial therapypt_PT
dc.subjectBioluminescencept_PT
dc.subjectEscherichia colipt_PT
dc.subjectSolar irradiationpt_PT
dc.titlePhotodynamic inactivation of recombinant bioluminescent Escherichia coli by cationic porphyrins under artificial and solar irradiationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1454pt_PT
oaire.citation.startPage1447pt_PT
oaire.citation.titleJournal of Industrial Microbiology & Biotechnologypt_PT
oaire.citation.volume35pt_PT
person.familyNameAlves
person.givenNameEliana
person.identifier.ciencia-idAB1A-4ABB-B29E
person.identifier.orcid0000-0001-5396-6536
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationc6b93be0-5472-434c-beab-722c303a29a8
relation.isAuthorOfPublication.latestForDiscoveryc6b93be0-5472-434c-beab-722c303a29a8

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