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An ultrasensitive human cardiac troponin T graphene screen-printed electrode based on electropolymerized-molecularly imprinted conducting polymer

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dc.contributor.authorSilva, Bárbara V.M.
dc.contributor.authorRodríguez, Blanca A.G.
dc.contributor.authorSales, Goreti
dc.contributor.authorSotomayor, Maria Del Pilar T.
dc.contributor.authorDutra, Rosa F.
dc.date.accessioned2017-07-13T11:15:22Z
dc.date.embargo2117
dc.date.issued2016
dc.description.abstractA nano-molecularly imprinted polymer (N-MIP) assembled on a screen-printed electrode for the cardiac troponin T (cTnT) was developed. The biomimetic surface was obtained by a co-polymer matrix as-sembled on the reduced graphene oxide (RGO) electrode surface. The cTnT active sites were engineered using pyrrole and carboxylated pyrrole that was one-step electropolymerized jointly with cTnT by cyclic voltammetry. The stepwise preparation of the biomimetic surface was characterized by cyclic and dif-ferential pulse voltammetries using the ferrocyanide/ferricyanide as redox probe. Structural and mor-phological characterization was also performed. The optimal relation of pyrrole and pyrrole-3-acid car-boxylic to perform the cTnT biomimetic nanosurface was obtained at 1:5 ratio. The analytical perfor-mance of cTnT N-MIP performed by differential pulse voltammetry showed a linear range from 0.01 to 0.1 ng mL-1 (r¼0.995, p«0.01), with a very low limit of detection (0.006 ng mL-1). The synergic effect of conductive polymer and graphene forming 3D structures of reactive sites resulted in a N-MIP with ex-cellent affinity to cTnT binding (KD¼7.3 10-13 mol L-1). The N-MIP proposed is based on a simple method of antibody obtaining with a large potential for point-of-care testing applications.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.bios.2015.10.068pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/10041
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relation.ispartofseriesBiosensors and Bioelectronics;Vol. 77
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0956566315305261pt_PT
dc.subjectCardiac troponin Tpt_PT
dc.subjectMolecularly imprinted polymerpt_PT
dc.subjectPyrrolept_PT
dc.subjectAcid carboxylic-3-pyrrolept_PT
dc.subjectGraphenept_PT
dc.subjectPoint-of-care testingpt_PT
dc.titleAn ultrasensitive human cardiac troponin T graphene screen-printed electrode based on electropolymerized-molecularly imprinted conducting polymerpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleBiosensors and Bioelectronicspt_PT
person.familyNameFerreira Sales
person.givenNameMaria Goreti
person.identifier826970
person.identifier.ciencia-idE31F-1630-42BB
person.identifier.orcid0000-0001-9936-7336
person.identifier.ridK-9199-2013
person.identifier.scopus-author-id7005174717
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb6d3a2d0-608a-4a92-834b-8d7b29069fef
relation.isAuthorOfPublication.latestForDiscoveryb6d3a2d0-608a-4a92-834b-8d7b29069fef

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