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Desenvolvimento de um sensor ótico para a deteção do biomarcador de cancro MMP7
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Abstract(s)
A doença oncológica tem mostrado uma tendência crescente nas últimas décadas, tornando-se
um problema de saúde pública. A falta de meios práticos e clinicamente confiáveis para a
monitorização do cancro representa um desafio no acompanhamento da doença em pacientes
que sofrem ou já sofreram desta condição. Para preencher essa lacuna, há a necessidade de se
desenvolverem novas estratégias sensoras para a deteção de biomarcadores associados a esta
doença. Um dos biomarcadores selecionado para o desenvolvimento deste trabalho, foi a
metaloproteinase de matriz 7 (MMP7, do inglês matrix metalloproteinase 7), conhecida por ser
expressa no cancro de pâncreas.
Neste contexto, desenvolveu-se um sensor biomimético denominado MIP@PQs, que consiste
na conjugação de polímeros molecularmente impressos (MIPs, do inglês, molecularly imprinted
polymers), com pontos quânticos (PQs). A construção do sensor utilizou PQs de telureto de
cádmio com emissão alaranjada, funcionalizados com ácido 3-mercaptopropiónico
(CdTe@MPA), que foram conjugados aos MIPs e utilizados na deteção do biomarcador MMP7
em soro humano liofilizado diluído a 1% em PBS (do inglês, phosphate buffer saline). Os
MIP@PQs apresentaram uma ampla faixa de deteção abaixo do valor de corte clínico para
MMP7, boa praticidade e seletividade em amostras de soro diluídas, o que indica o seu
potencial para várias aplicações biológicas.
Considerando o desempenho promissor do sensor biomimético em solução, planeia-se fazer a
sua deposição em suporte sólido e avaliar a resposta analítica ao biomarcador MMP7,
constituindo uma potencial linha de investigação para a continuação deste estudo.
Oncological disease has shown a rising trend over the past decades, becoming a major public health concern. The lack of practical and clinically reliable methods for cancer monitoring presents a challenge in tracking the disease in patients currently affected or who have had this condition. To address this gap, it is necessary to develop new sensing strategies for the detection of biomarkers associated with cancer. One of the biomarkers selected for this work was matrix metalloproteinase 7 (MMP7), known for its expression in pancreatic cancer. In this context, a biomimetic sensor was developed based on molecularly imprinted polymers (MIPs) combined with quantum dots, referred to as MIP@PQs. The sensor construction utilized orange-emitting cadmium telluride quantum dots functionalized with 3-mercaptopropionic acid (CdTe@MPA), which were conjugated with MIPs and used in detecting the biomarker MMP7 in lyiohilized human serum 1% diluted in phosphate buffer saline, PBS. The MIP@PQs exhibited a broad detection range below the clinical cutoff for MMP7, demonstrating excellent practicality and selectivity in diluted serum samples, indicating its potential for various biological applications Given the promising performance of the biomimetic sensor in solution, the next step involves its deposition onto a solid support to evaluate its analytical response to the MMP7 biomarker. This approach opens a potential avenue for future research to continue this study.
Oncological disease has shown a rising trend over the past decades, becoming a major public health concern. The lack of practical and clinically reliable methods for cancer monitoring presents a challenge in tracking the disease in patients currently affected or who have had this condition. To address this gap, it is necessary to develop new sensing strategies for the detection of biomarkers associated with cancer. One of the biomarkers selected for this work was matrix metalloproteinase 7 (MMP7), known for its expression in pancreatic cancer. In this context, a biomimetic sensor was developed based on molecularly imprinted polymers (MIPs) combined with quantum dots, referred to as MIP@PQs. The sensor construction utilized orange-emitting cadmium telluride quantum dots functionalized with 3-mercaptopropionic acid (CdTe@MPA), which were conjugated with MIPs and used in detecting the biomarker MMP7 in lyiohilized human serum 1% diluted in phosphate buffer saline, PBS. The MIP@PQs exhibited a broad detection range below the clinical cutoff for MMP7, demonstrating excellent practicality and selectivity in diluted serum samples, indicating its potential for various biological applications Given the promising performance of the biomimetic sensor in solution, the next step involves its deposition onto a solid support to evaluate its analytical response to the MMP7 biomarker. This approach opens a potential avenue for future research to continue this study.
Description
Keywords
Molecularly imprinted polymers quantum dots biomimetic optic sensor MIP@PQs pancreatic cancer MMP7 biomarker Polímeros de impressão molecular Pontos quânticos Sensor ótico biomimético MIP@POs Cancro do Pâncreas Biomarcador MMP7