Repository logo
 
Publication

Influence of the metabolic state on the tolerance of Pichia kudriavzevii to heavy metals

2016Journal article Restricted access
Simple item page
dc.contributor.authorMesquita, Vanessa A.
dc.contributor.authorMachado, Manuela D.
dc.contributor.authorSilva, Cristina F.
dc.contributor.authorSoares, Eduardo V.
dc.date.accessioned2017-01-09T15:21:33Z
dc.date.embargo2117
dc.date.issued2016
dc.description.abstractThis work aims to examine the influence of the metabolic state of the yeast Pichia kudriavzevii on the susceptibility to a metals mixture (5 mg L(-1) Cd, 10 mg L(-1) Pb, and 5 mg L(-1) Zn). Cells exposed to the metals mixture in the presence of 25 mmol L(-1) glucose displayed a higher loss of membrane integrity and proliferation capacity, compared to cells incubated in the absence of glucose. The analysis of the effect of individual metals revealed that glucose increased the toxic effect of Cd marginally, and of Pb significantly. The increased susceptibility to heavy metals due to glucose was attenuated in the simultaneous presence of a mitochondrial respiration inhibitor such as sodium azide (NaN3 ). ATP-depleted yeast cells, resulting from treatment with the non-metabolizable glucose analogue 2-deoxy-d-glucose, showed an increased susceptibility to heavy metals mixture. Pre-incubation of yeast cells with 1 or 1.5 mmol L(-1) Ca(2+) reduced significantly (P < 0.05) the loss of membrane integrity induced by the metals mixture. These findings contribute to the understanding of metals mechanisms of toxicity in the non-conventional yeast P. kudriavzevii.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1002/jobm.201600232pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/9169
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWILEY-VCH Verlagpt_PT
dc.relation.ispartofseriesJournal of Basic Microbiology;Vol. 56, Issue 11
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1002/jobm.201600232/abstract;jsessionid=66C6C8C1E61D607A2A8CCC3E74E3414A.f03t02?systemMessage=Wiley+Online+Library+Journal+subscribe+and+renew+pages+for+some+journals+will+be+unavailable+on+Wednesday+11th+January+2017+from+06%3A00-12%3A00+GMT+%2F+01%3A00-07%3A00+EST+%2F+14%3A00-20%3A00+SGT+for+essential+maintenance.+Apologies+for+the+inconveniencept_PT
dc.subject2-deoxy-D-glucose (2-DOG)pt_PT
dc.subjectHeavy metals toxicitypt_PT
dc.subjectMembrane integritypt_PT
dc.subjectSodium azidept_PT
dc.subjectYeast viabilitypt_PT
dc.titleInfluence of the metabolic state on the tolerance of Pichia kudriavzevii to heavy metalspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1251pt_PT
oaire.citation.issue11pt_PT
oaire.citation.startPage1244pt_PT
oaire.citation.titleJournal of Basic Microbiologypt_PT
oaire.citation.volume56pt_PT
person.familyNameSoares
person.givenNameEduardo
person.identifier93637
person.identifier.ciencia-idD612-0D0E-4984
person.identifier.orcid0000-0003-2280-5291
person.identifier.ridO-5908-2015
person.identifier.scopus-author-id7102060173
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb3ebdee1-84fa-4183-a1d8-c3cdcaaef219
relation.isAuthorOfPublication.latestForDiscoveryb3ebdee1-84fa-4183-a1d8-c3cdcaaef219

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
ART2_EVS_CIETI_2016.pdf
Size:
415.52 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ISEP – CIETI – Artigos