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Insights on Ultrafiltration-Based Separation for the Purification and Quantification of Methotrexate in Nanocarriers

2020-04Journal article Open access
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dc.contributor.authorMarques, Sara S.
dc.contributor.authorRamos, Inês I.
dc.contributor.authorFernandes, Sara
dc.contributor.authorBarreiros, Luisa
dc.contributor.authorLima, Sofia A. C.
dc.contributor.authorReis, Salette
dc.contributor.authorDomingues, M. Rosário M.
dc.contributor.authorSegundo, Marcela A.
dc.date.accessioned2020-06-19T09:28:14Z
dc.date.available2020-06-19T09:28:14Z
dc.date.issued2020-04
dc.description.abstractThe evaluation of encapsulation efficiency is a regulatory requirement for the characterization of drug delivery systems. However, the difficulties in efficiently separating nanomedicines from the free drug may compromise the achievement of accurate determinations. Herein, ultrafiltration was exploited as a separative strategy towards the evaluation of methotrexate (MTX) encapsulation efficiency in nanostructured lipid carriers and polymeric nanoparticles. The effect of experimental conditions such as pH and the amount of surfactant present in the ultrafiltration media was addressed aiming at the selection of suitable conditions for the effective purification of nanocarriers. MTX-loaded nanoparticles were then submitted to ultrafiltration and the portions remaining in the upper compartment of the filtering device and in the ultrafiltrate were collected and analyzed by HPLC-UV using a reversed-phase (C18) monolithic column. A short centrifugation time (5 min) was suitable for establishing the amount of encapsulated MTX in nanostructured lipid carriers, based on the assumption that the free MTX concentration was the same in the upper compartment and in the ultrafiltrate. The defined conditions allowed the efficient separation of nanocarriers from the free drug, with recoveries of >85% even when nanoparticles were present in cell culture media and in pig skin surrogate from permeation assays.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/molecules25081879pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/16000
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationLISBOA-01-0145-FEDER-402-022125pt_PT
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationNot available
dc.relationTotal analysis systems based on mesofluidics coupled to tandem mass spectrometry
dc.relationOrganic Chemistry, Natural Products and Food Stuffs
dc.relationCentre for Environmental and Marine Studies
dc.relation.publisherversionhttps://www.mdpi.com/1420-3049/25/8/1879pt_PT
dc.subjectDrug deliverypt_PT
dc.subjectUltrafiltrationpt_PT
dc.subjectEncapsulation efficiencypt_PT
dc.subjectSeparative methodspt_PT
dc.subjectPermeationpt_PT
dc.titleInsights on Ultrafiltration-Based Separation for the Purification and Quantification of Methotrexate in Nanocarrierspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardTitleNot available
oaire.awardTitleTotal analysis systems based on mesofluidics coupled to tandem mass spectrometry
oaire.awardTitleOrganic Chemistry, Natural Products and Food Stuffs
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//PD%2FBD%2F128398%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F97540%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F130948%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FQUI%2F00062%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FAMB%2F50017%2F2019/PT
oaire.citation.issue8pt_PT
oaire.citation.startPage1879pt_PT
oaire.citation.titleMoleculespt_PT
oaire.citation.volume25pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamSFRH
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameFernandes
person.familyNameBarreiros
person.givenNameSara
person.givenNameLuisa
person.identifier.ciencia-id1C12-D800-38A4
person.identifier.ciencia-id611F-E0C5-0230
person.identifier.orcid0000-0001-7042-1941
person.identifier.orcid0000-0003-3481-5809
person.identifier.ridD-7950-2013
person.identifier.scopus-author-id57203278917
person.identifier.scopus-author-id6508205485
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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