Publication
New approach for vitamin E extraction in rainbow trout flesh: Application in fish fed commercial and red seaweed-supplemented diets
| dc.contributor.author | Araújo, Mariana | |
| dc.contributor.author | Alves, Rita C. | |
| dc.contributor.author | Pimentel, Filipa B. | |
| dc.contributor.author | Costa, Anabela S.G. | |
| dc.contributor.author | Fernandes, Telmo J.R. | |
| dc.contributor.author | Valente, Luísa M.P. | |
| dc.contributor.author | Rema, Paulo | |
| dc.contributor.author | Oliveira, M. Beatriz P.P. | |
| dc.date.accessioned | 2015-12-23T11:40:12Z | |
| dc.date.available | 2015-12-23T11:40:12Z | |
| dc.date.issued | 2015-06 | |
| dc.description.abstract | A vitamin E extraction method for rainbow trout flesh was optimized, validated, and applied in fish fed commercial and Gracilaria vermiculophylla-supplemented diets. Five extraction methods were compared. Vitamers were analyzed by HPLC/DAD/fluorescence. A solid-liquid extraction with n-hexane, which showed the best performance, was optimized and validated. Among the eight vitamers, only α- and γ-tocopherol were detected in muscle samples. The final method showed good linearity (>0.999), intra- (<3.1%) and inter-day precision (<2.6%), and recoveries (>96%). Detection and quantification limits were 39.9 and 121.0 ng/g of muscle, for α-tocopherol, and 111.4 ng/g and 337.6 ng/g, for γ-tocopherol, respectively. Compared to the control group, the dietary inclusion of 5% G. vermiculophylla resulted in a slight reduction of lipids in muscle and, consequently, of α- and γ-tocopherol. Nevertheless, vitamin E profile in lipids was maintained. In general, the results may be explained by the lower vitamin E level in seaweed-containing diet. Practical Applications: Based on the validation results and the low solvent consumption, the developed method can be used to analyze vitamin E in rainbow trout. The results of this work are also a valuable information source for fish feed industries and aquaculture producers, which can focus on improving seaweed inclusion in feeds as a source of vitamin E in fish muscle and, therefore, take full advantage of all bioactive components with an important role in fish health and flesh quality. | pt_PT |
| dc.identifier.doi | 10.1002/ejlt.201400656 | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10400.22/7236 | |
| dc.language.iso | eng | pt_PT |
| dc.publisher | WILEY-VCH | pt_PT |
| dc.relation.ispartofseries | European Journal of Lipid Science and Technology;Vol. 117, Issue 9 | |
| dc.relation.publisherversion | http://onlinelibrary.wiley.com/doi/10.1002/ejlt.201400656/full | pt_PT |
| dc.subject | Vitamin E | pt_PT |
| dc.subject | Rainbow trout flesh | pt_PT |
| dc.subject | Gracilaria vermiculophylla | pt_PT |
| dc.subject | Feed | pt_PT |
| dc.subject | Extraction method | pt_PT |
| dc.title | New approach for vitamin E extraction in rainbow trout flesh: Application in fish fed commercial and red seaweed-supplemented diets | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 1405 | pt_PT |
| oaire.citation.startPage | 1398 | pt_PT |
| oaire.citation.title | European Journal of Lipid Science and Technology | pt_PT |
| oaire.citation.volume | 117 | pt_PT |
| person.familyName | Alves | |
| person.givenName | Rita C. | |
| person.identifier.orcid | 0000-0002-5053-513X | |
| person.identifier.rid | I-9081-2012 | |
| person.identifier.scopus-author-id | 7102691286 | |
| rcaap.rights | closedAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isAuthorOfPublication | 4b5cf5a1-8445-4d6a-ac30-5fbee0326269 | |
| relation.isAuthorOfPublication.latestForDiscovery | 4b5cf5a1-8445-4d6a-ac30-5fbee0326269 |