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Saccharomyces cerevisiae Mutants Affected in Vacuole Assembly or Vacuolar H+-ATPase are Hypersensitive to Lead (Pb) Toxicity

dc.contributor.authorSousa, Cátia A.
dc.contributor.authorPerez, Rita R.
dc.contributor.authorSoares, Eduardo V.
dc.date.accessioned2015-01-05T12:16:25Z
dc.date.available2015-01-05T12:16:25Z
dc.date.issued2014
dc.description.abstractLead is an important environmental pollutant. The role of vacuole, in Pb detoxification, was studied using a vacuolar protein sorting mutant strain (vps16D), belonging to class C mutants. Cells disrupted in VPS16 gene, did not display a detectable vacuolar-like structure. Based on the loss of cell proliferation capacity, it was found that cells from vps16D mutant exhibited a hypersensitivity to Pb-induced toxicity, compared to wild type (WT) strain. The function of vacuolar H?-ATPase (VATPase), in Pb detoxification, was evaluated using mutants with structurally normal vacuoles but defective in subunits of catalytic (vma1D or vma2D) or membrane domain (vph1D or vma3D) of V-ATPase. All mutants tested, lacking a functional V-ATPase, displayed an increased susceptibility to Pb, comparatively to cells from WT strain. Modification of vacuolar morphology, in Pb-exposed cells, was visualized using a Vma2p-GFP strain. The treatment of yeast cells with Pb originated the fusion of the medium size vacuolar lobes into one enlarged vacuole. In conclusion, it was found that vacuole plays an important role in the detoxification of Pb in Saccharomyces cerevisiae; in addition, a functional V-ATPase was required for Pb compartmentalization.por
dc.identifier.doi10.1007/s00284-013-0438-y
dc.identifier.urihttp://hdl.handle.net/10400.22/5278
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringerpor
dc.relation.ispartofseriesCurrent Microbiology;Vol. 68, Issue 1
dc.relation.publisherversionhttp://link.springer.com/article/10.1007%2Fs00284-013-0438-ypor
dc.subjectMetals toxicitypor
dc.subjectPb detoxificationpor
dc.subjectVacuolar H+-ATPasepor
dc.subjectYeastpor
dc.titleSaccharomyces cerevisiae Mutants Affected in Vacuole Assembly or Vacuolar H+-ATPase are Hypersensitive to Lead (Pb) Toxicitypor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage119por
oaire.citation.startPage113por
oaire.citation.titleCurrent Microbiologypor
oaire.citation.volume68por
person.familyNameSoares
person.givenNameEduardo
person.identifier93637
person.identifier.ciencia-idD612-0D0E-4984
person.identifier.orcid0000-0003-2280-5291
person.identifier.ridO-5908-2015
person.identifier.scopus-author-id7102060173
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationb3ebdee1-84fa-4183-a1d8-c3cdcaaef219
relation.isAuthorOfPublication.latestForDiscoveryb3ebdee1-84fa-4183-a1d8-c3cdcaaef219

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