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Disclosure of a promising lead to tackle complicated skin and skin structure infections: antimicrobial and antibiofilm actions of peptide PP4-3.1

2021-11-19Journal article Open access
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dc.contributor.authorGomes, Ana
dc.contributor.authorBessa, Lucinda J.
dc.contributor.authorFernandes, Iva
dc.contributor.authorFerraz, Ricardo
dc.contributor.authorMonteiro, Cláudia
dc.contributor.authorMartins, M. Cristina L.
dc.contributor.authorMateus, Nuno
dc.contributor.authorGameiro, Paula
dc.contributor.authorTeixeira, Cátia
dc.contributor.authorGomes, Paula
dc.date.accessioned2022-04-28T12:02:12Z
dc.date.available2022-04-28T12:02:12Z
dc.date.issued2021-11-19
dc.description.abstractEfficient antibiotics are being exhausted, which compromises the treatment of infections, including complicated skin and skin structure infections (cSSTI) often associated with multidrug resistant (MDR) bacteria, methicillin-resistant S. aureus (MRSA) being the most prevalent. Antimicrobial peptides (AMP) are being increasingly regarded as the new hope for the post-antibiotic era. Thus, future management of cSSTI may include use of peptides that, on the one hand, behave as AMP and, on the other, are able to promote fast and correct skin rebuilding. As such, we combined the well-known cosmeceutical pentapeptide-4 (PP4), devoid of antimicrobial action but possessing collagenesis-boosting properties, with the AMP 3.1, to afford the chimeric peptide PP4-3.1. We further produced its N-methyl imidazole derivative, MeIm-PP4-3.1. Both peptide-based constructs were evaluated in vitro against Gram-negative bacteria, Gram-positive bacteria, and Candida spp. fungi. Additionally, the antibiofilm activity, the toxicity to human keratinocytes, and the activity against S. aureus in simulated wound fluid (SWF) were assessed. The chimeric peptide PP4-3.1 stood out for its potent activity against Gram-positive and Gram-negative bacteria, including against MDR clinical isolates (0.8 ≤ MIC ≤ 5.7 µM), both in planktonic form and in biofilm matrix. The peptide was also active against three clinically relevant species of Candida fungi, with an overall performance superior to that of fluconazole. Altogether, data reveal that PP4-3.1 is as a promising lead for the future development of new topical treatments for severe skin infections.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGomes, A., Bessa, L. J., Fernandes, I., Ferraz, R., Monteiro, C., L. Martins, M. C., Mateus, N., Gameiro, P., Teixeira, C., & Gomes, P. (2021). Disclosure of a Promising Lead to Tackle Complicated Skin and Skin Structure Infections: Antimicrobial and Antibiofilm Actions of Peptide PP4-3.1. Pharmaceutics, 13(11), 1962. https://www.mdpi.com/1999-4923/13/11/1962pt_PT
dc.identifier.doi10.3390/pharmaceutics13111962pt_PT
dc.identifier.eissn1999-4923
dc.identifier.urihttp://hdl.handle.net/10400.22/20416
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttps://www.mdpi.com/1999-4923/13/11/1962pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAntibacterialpt_PT
dc.subjectAntibiofilmpt_PT
dc.subjectAntifungalpt_PT
dc.subjectAntimicrobial peptidespt_PT
dc.subjectCollagenpt_PT
dc.subjectMultidrug resistancept_PT
dc.subjectSkin infectionspt_PT
dc.subjectWound healingpt_PT
dc.titleDisclosure of a promising lead to tackle complicated skin and skin structure infections: antimicrobial and antibiofilm actions of peptide PP4-3.1pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage15pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titlePharmaceuticspt_PT
oaire.citation.volume13pt_PT
person.familyNameFerraz
person.givenNameRicardo
person.identifier.ciencia-id001E-71CE-F92D
person.identifier.orcid0000-0002-1761-117X
person.identifier.ridG-5639-2011
person.identifier.scopus-author-id24464208500
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationa5a8faa7-12a5-4b1c-bced-44c895677397
relation.isAuthorOfPublication.latestForDiscoverya5a8faa7-12a5-4b1c-bced-44c895677397

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