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Warburg Effect Inversion: Adiposity shifts central primary metabolism in MCF-7 breast cancer cells

2019Journal article Open access
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dc.contributor.authorLuís, Carla
dc.contributor.authorDuarte, Fernanda
dc.contributor.authorFaria, Isabel
dc.contributor.authorJarak, Ivana
dc.contributor.authorOliveira, Pedro F.
dc.contributor.authorAlves, Marco G.
dc.contributor.authorSoares, Raquel
dc.contributor.authorFernandes, Rúben
dc.date.accessioned2019-06-28T15:45:11Z
dc.date.available2023-05-02T00:31:39Z
dc.date.issued2019
dc.description.abstractObesity is a complex health disorder and a trigger to many diseases like Diabetes mellitus (DM) and breast cancer (BrCa), both leading causes of morbidity and mortality worldwide. Also evidence demonstrates that abnormal glucose metabolism termed ‘the Warburg effect’ in cancer cell is closely associated with malignant phenotypes and promote the aggressiveness of several types of cancer, including BrCa. In this study, we evaluated the breast cancer cell metabolism in normoglycemia, hyperglycemia and in an obesity condition in order to clarify the potential underlined mechanisms that link these disorders. Materials and methods MCF-7 cells were exposed to low and high glucose levels, the latter either in the presence of 3T3-L1 adipocyte conditioned medium (CM), thus mimicking the adiposity observed in obese patients. Cell viability, migration, proliferation, cytotoxicity and cell death assays were performed under the different culture conditions. Hormonal and lipid profile were also characterized by biochemical assays and primary metabolism was determined by Nuclear Magnetic Resonance (NMR)-based metabolomics. Results Our results show an increased aggressiveness in the condition mimicking diabetogenic obesity with an altered energy/lipid metabolism. Interestingly in the experimental obesity-mimicking status, lipids and amino acids were expended while glucose was produced by tumor cells from lactate. These findings reveal a shift on tumor cells metabolism that is opposite to ‘the Warburg effect’. Conclusions Overall, this experimentally obesity-mimicking condition not only revealed an increased tumor proliferation and aggressiveness but also disclosed a new mechanism of cancer metabolism, the ‘Warburg Effect Inversion’.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLuis, C., Duarte, F., Faria, I., Jarak, I., Oliveira, P. F., Alves, M. G., Soares, R., & Fernandes, R. (2019). Warburg Effect Inversion: Adiposity shifts central primary metabolism in MCF-7 breast cancer cells. Life Sciences, 223, 38–46. https://doi.org/10.1016/j.lfs.2019.03.016
dc.identifier.doi10.1016/j.lfs.2019.03.016pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/14163
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationNORTE-01-0145-FEDER-000012pt_PT
dc.relationEarly detection and monitoring of metabolic progression of type 2 diabetes
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0024320519301742pt_PT
dc.subjectType 2 diabetespt_PT
dc.subjectAdipocytespt_PT
dc.subjectMCF-7 Cellspt_PT
dc.subjectBreast Cancerpt_PT
dc.subjectWarburg effectpt_PT
dc.subjectMetabolismpt_PT
dc.titleWarburg Effect Inversion: Adiposity shifts central primary metabolism in MCF-7 breast cancer cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleEarly detection and monitoring of metabolic progression of type 2 diabetes
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIM%2F04293%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9876 - Politécnicos/SAICT-POL%2F24325%2F2016/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9876 - Politécnicos/SAICT-POL%2F24358%2F2016/PT
oaire.citation.endPage46pt_PT
oaire.citation.startPage38pt_PT
oaire.citation.titleLife Sciencespt_PT
oaire.citation.volume223pt_PT
oaire.fundingStream5876
oaire.fundingStream9876 - Politécnicos
oaire.fundingStream9876 - Politécnicos
person.familyNameDuarte
person.familyNameFernandes
person.givenNameFernanda
person.givenNameRúben
person.identifier635792
person.identifier.ciencia-idF411-11C2-BA48
person.identifier.ciencia-id0D1F-4090-E82A
person.identifier.orcid0000-0001-8933-3984
person.identifier.scopus-author-id57640135700
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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