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Passive direct methanol fuel cells acting as fully autonomous electrochemical biosensors: Application to sarcosine detection

2022Journal article Open access
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dc.contributor.authorSilva Ferreira, Nadia
dc.contributor.authorCarneiro, Liliana P.T
dc.contributor.authorViezzer, Christian
dc.contributor.authorAlmeida, Maria J.T.
dc.contributor.authorMarques, Ana C.
dc.contributor.authorPinto, Alexandra M.F.R.
dc.contributor.authorFortunato, Elvira
dc.contributor.authorFerreira Sales, Maria Goreti
dc.date.accessioned2023-01-26T09:59:00Z
dc.date.available2023-01-26T09:59:00Z
dc.date.issued2022
dc.description.abstractThis work describes an innovative electrochemical biosensor that advances its autonomy toward an equipment-free design. The biosensor is powered by a passive direct methanol fuel cell (DMFC) and signals the response via an electrochromic display. Briefly, the anode side of the DMFC power source was modified with a biosensor layer developed using molecularly imprinted polymer (MIP) technology to detect sarcosine (an amino acid derivative that is a potential cancer biomarker). The biosensor layer was anchored on the surface of the anode carbon electrode (carbon black with Pt/Ru, 40:20). This was done by bulk radical polymerization with acrylamide, bis-acrylamide, and vinyl phosphonic acid. This layer selectively interacted with sarcosine when integrated into the passive DMFC (single or multiple, in a stack of 4), which acted as a transducer element in a concentration-dependent process. Serial assembly of a stack of hybrid DMFC/biosensor devices triggered an external electrochromic cell (EC) that produced a colour change. Calibrations showed a concentration-dependent sarcosine response from 3.2 to 2000 µM, which is compatible with the concentration of sarcosine in the blood of prostate cancer patients. The final DMFC/biosensor-EC platform showed a colour change perceptible to the naked eye in the presence of increasing sarcosine concentrations. This colour change was controlled by the DMFC operation, making this approach a self-controlled and self-signalling device. Overall, this approach is a proof-of-concept for a fully autonomous biosensor powered by a chemical fuel. This simple and low-cost approach offers the potential to be deployed anywhere and is particularly suitable for point-of-care (POC) analysis.pt_PT
dc.description.sponsorshipThe authors acknowledge the financial support of EU-Horizon 2020 (Symbiotic, FET-Open, GA665046), and from national funds from FCT - Fundação para a Ciência e a Tecnologia, I.P., in the scope of the projects LA/P/0037/2020, UIDP/50025/2020, UIDB/50025/2020 and UID/EMS/00532/2019. Nádia Ferreira (SFRH/BD/122955/2016), Liliana Carneiro (SFRH/BD/122954/2016), and Ana Carolina Marques (SFRH/BD/115173/2016) acknowledge Fundação para a Ciência e Tecnologia (FCT) for financial support.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.jelechem.2022.116710pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/21879
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relationLA/P/0037/2020pt_PT
dc.relationINNOVATIVE AUTONOMOUS ELECTRICAL BIOSENSOR SYNERGISTICALLY ASSEMBLED INSIDE A PASSIVE DIRECT METHANOL FUEL CELL FOR SCREENING CANCER BIOMARKERS
dc.relationInstitute of Nanostructures, Nanomodelling and Nanofabrication
dc.relationInstitute of Nanostructures, Nanomodelling and Nanofabrication
dc.relationStudy Center of Transport Phenomena
dc.relationAutonomous biosensor for screening cancer biomarkers using passive Direct Methanol Fuel Cells
dc.relationA new vision for fuel cells: application in cancer biomarker screening
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S1572665722007020?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectPassive direct methanol fuel cellpt_PT
dc.subjectMolecularly imprinted polymerpt_PT
dc.subjectElectrochromic cellpt_PT
dc.subjectColour displaypt_PT
dc.subjectSarcosinept_PT
dc.subjectCancer biomarkerpt_PT
dc.titlePassive direct methanol fuel cells acting as fully autonomous electrochemical biosensors: Application to sarcosine detectionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleINNOVATIVE AUTONOMOUS ELECTRICAL BIOSENSOR SYNERGISTICALLY ASSEMBLED INSIDE A PASSIVE DIRECT METHANOL FUEL CELL FOR SCREENING CANCER BIOMARKERS
oaire.awardTitleInstitute of Nanostructures, Nanomodelling and Nanofabrication
oaire.awardTitleInstitute of Nanostructures, Nanomodelling and Nanofabrication
oaire.awardTitleStudy Center of Transport Phenomena
oaire.awardTitleAutonomous biosensor for screening cancer biomarkers using passive Direct Methanol Fuel Cells
oaire.awardTitleA new vision for fuel cells: application in cancer biomarker screening
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/665046/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50025%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50025%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FEMS%2F00532%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F122955%2F2016/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F122954%2F2016/PT
oaire.citation.startPage116710pt_PT
oaire.citation.titleJournal of Electroanalytical Chemistrypt_PT
oaire.citation.volume922pt_PT
oaire.fundingStreamH2020
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamPOR_NORTE
person.familyNameSilva Ferreira
person.familyNameTeixeira Carneiro
person.familyNameViezzer
person.familyNameMarques
person.familyNameFerreira Rodrigues Pinto
person.familyNameCorreia Fortunato
person.familyNameFerreira Sales
person.givenNameNadia
person.givenNameLiliana Patrícia
person.givenNameChristian
person.givenNameAna C.
person.givenNameAlexandra
person.givenNameElvira Maria
person.givenNameMaria Goreti
person.identifierhttps://scholar.google.pt/citations?user=aH1LTDwAAAAJ&hl=pt-PT
person.identifier826970
person.identifier.ciencia-id4310-FE12-C862
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person.identifier.ciencia-id7518-FD35-9DFD
person.identifier.ciencia-idDE1F-9132-B3FF
person.identifier.ciencia-idE31F-1630-42BB
person.identifier.orcid0000-0001-8251-1223
person.identifier.orcid0000-0003-2800-7418
person.identifier.orcid0000-0001-9886-8745
person.identifier.orcid0000-0002-2937-5186
person.identifier.orcid0000-0002-4202-7047
person.identifier.orcid0000-0001-9936-7336
person.identifier.ridD-1119-2019
person.identifier.ridK-9199-2013
person.identifier.scopus-author-id55888120300
person.identifier.scopus-author-id31367723800
person.identifier.scopus-author-id35420037600
person.identifier.scopus-author-id7005174717
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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