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Flow-through 3D-printed device for automatic microsampling and handling of dried urine spots

2026-02-01Artigo de investigação Acesso embargado
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dc.contributor.authorFernandes, Sara R.
dc.contributor.authorCunha, Diana R.
dc.contributor.authorGuidetti, Federica
dc.contributor.authorBarreiros, Luísa
dc.contributor.authorMiró, Manuel
dc.contributor.authorSegundo, Marcela A.
dc.contributor.authorBarreiros, Luisa
dc.contributor.authorFernandes, Sara
dc.date.accessioned2026-05-29T14:14:24Z
dc.date.available2026-05-29T14:14:24Z
dc.date.issued2026-02-01
dc.description.abstract3D printing has revolutionized analytical chemistry by allowing the development of miniaturized and high- precision devices. In bioanalysis, sample collection and pre-treatment can be facilitated using 3D printing combined with flow analysis and mass spectrometry. Hence, a customized 3D-printed device was designed for sampling, clean-up, and target retrieval using filter paper as a sample collection material and sorbent. This device was integrated into a flow network for fully automatic extraction and applied to detect three anticoagulants in human urine. Different printing materials, designs and other factors related to fused deposition modeling 3D printing such as the infill percentage were evaluated to achieve the configuration that allowed the implementation of sampling and separation procedures in the same device. After establishing the final design of the device, several parameters such as the eluent composition and the hydrodynamic conditions were studied to enhance the recovery of the target analytes, namely apixaban, rivaroxaban, and warfarin. The processed samples were analyzed by UHPLC-MS/MS in positive ionization mode using a BEH C18 column. The method demonstrated good linearity (r2 >0.998) for quantification of the target analytes at concentrations ranging from 0.20 to 20 μg L 1 for apixaban and warfarin, and from 0.50 to 20 μg L 1 for rivaroxaban. LOD and LOQ values of 0.06–0.2 μg L 1 and 0.1–0.5 μg L 1, respectively, for undiluted urine were obtained. The method was found to be accurate (97.0–102 %) and precise (CV ≤6.0 %). This new approach, according to the scores obtained by applying the AGREEprep (0.70), AGREE (0.65) and BAGI (70) metrics, can be described as environmentally friendly, practical and suitable for bioanalytical purposes.eng
dc.description.sponsorshipPID2020-117686RB-C33-R and PID2024-156804OBC33 (MCIN/AEI/10.13039/501100011033)
dc.identifier.citationFernandes, S. R., Cunha, D. R., Guidetti, F., Barreiros, L., Miró, M., & Segundo, M. A. (2026). Flow-through 3D-printed device for automatic microsampling and handling of dried urine spots. Talanta, 298, 129030. https://doi.org/10.1016/j.talanta.2025.129030
dc.identifier.doi10.1016/j.talanta.2025.129030
dc.identifier.eissn2666-8319
dc.identifier.urihttp://hdl.handle.net/10400.22/32470
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationUID/50006/2025; 2022.06012.PTDC; SFRH/BD/130948/2017, COVID/BD/ 152406/2022 and 2020.04917.BD
dc.relation.hasversionhttps://www.sciencedirect.com/science/article/pii/S0039914025015218?via%3Dihub
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject3D printing
dc.subjectAutomation
dc.subjectDried urine spot
dc.subjectFlow analysis
dc.subjectMass spectrometry
dc.titleFlow-through 3D-printed device for automatic microsampling and handling of dried urine spotseng
dc.typeresearch article
dspace.entity.typePublication
oaire.citation.issuePart B
oaire.citation.titleTalanta
oaire.citation.volume298
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameBarreiros
person.familyNameFernandes
person.givenNameLuisa
person.givenNameSara
person.identifier.ciencia-id611F-E0C5-0230
person.identifier.ciencia-id1C12-D800-38A4
person.identifier.orcid0000-0003-3481-5809
person.identifier.orcid0000-0001-7042-1941
person.identifier.ridD-7950-2013
person.identifier.scopus-author-id6508205485
person.identifier.scopus-author-id57203278917
relation.isAuthorOfPublication1e66bacc-64de-4ecb-96b7-4c0e366cba57
relation.isAuthorOfPublication6c2ed5f0-c3fd-4559-99a3-b8d78d019b47
relation.isAuthorOfPublication.latestForDiscovery1e66bacc-64de-4ecb-96b7-4c0e366cba57

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