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Paper-based ELISA for fast CA 15–3 detection in point-of-care

2022Journal article Restricted access
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dc.contributor.authorCarneiro, Mariana C.C.G.
dc.contributor.authorRodrigues, R. V.
dc.contributor.authorMoreira, Felismina
dc.contributor.authorSales, Maria Goreti Ferreira
dc.date.accessioned2023-01-25T16:37:36Z
dc.date.embargo2035
dc.date.issued2022
dc.description.abstractThe paper-based Enzyme-Linked Immunosorbent Assay (P-ELISA) is a promising tool for diagnostic applications because the paper matrix is characterised by low cost, short analysis time, portability, and a high surface-to-volume ratio that allows the use of a small sample and reagent volume of a few microliters. In addition, colorimetric assays are suitable for low-resource areas due to their simplicity and naked-eye detectability. Although several works have been reported using paper-based colorimetric sensors for cancer biomarkers, P-ELISA for cancer antigen 15–3 (CA 15–3) has never been reported. Thus, this work reports the development of a rapid, simple and relatively inexpensive paper-based colorimetric assay for the detection of CA 15–3 as cancer biomarker. The assay was developed on a filter paper that was previously washed and chemically oxidized with periodate to generate aldehyde functional groups on the cellulose surface. After covalent binding of the first antibody, detection was performed by a colorimetric reaction based on the oxidation of the substrate 3,3′,5,5′-tetramethylbenzidine (TMB) by a peroxidase enzyme in the presence of H2O2. The colorimetric measurement was based on digital image acquisition analysed using ImageJ software. The linear range of the assay in buffer ranged 2 to 1100 U/mL. The performance of the assay was also successfully tested in human serum from Cormay®, offering a linear range from 2 to 200 U/mL. Thus, this P-ELISA sensor is suitable for the analysis of serum samples, since the physiological value in cancer patients is 30 U/mL. We believe that this proof-of-concept has the potential to be extended to be applied to other protein biomarkers and to be a suitable tool for cancer screening in developing countries.pt_PT
dc.description.sponsorshipThis work was supported by project TecniCOV (POCI-01-02B7- FEDER-069745) and funding by Fundação para a Ciência e Tecnologia, I.P., through the PhD grant reference SFRH/BD/131959/2017 and the strategic funding of UIDB/04469/2020 unit.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.microc.2022.107756pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/21869
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relationPOCI-01-02B7- FEDER-069745pt_PT
dc.relationTest-strips for monitoring cancer biomarkers in point-of-care
dc.relationCentre of Biological Engineering of the University of Minho
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0026265X22005847?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectPaper-based ELISApt_PT
dc.subjectCA 15–3pt_PT
dc.subjectColorimetric detectionpt_PT
dc.subjectCancer detectionpt_PT
dc.subjectBreast cancerpt_PT
dc.titlePaper-based ELISA for fast CA 15–3 detection in point-of-carept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTest-strips for monitoring cancer biomarkers in point-of-care
oaire.awardTitleCentre of Biological Engineering of the University of Minho
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F131959%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT
oaire.citation.startPage107756pt_PT
oaire.citation.titleMicrochemical Journalpt_PT
oaire.citation.volume181pt_PT
oaire.fundingStreamPOR_NORTE
oaire.fundingStream6817 - DCRRNI ID
person.familyNameRodrigues
person.familyNameMoreira
person.familyNameFerreira Sales
person.givenNameRaul
person.givenNameFelismina
person.givenNameMaria Goreti
person.identifier1589429
person.identifier826970
person.identifier.ciencia-idC917-6A46-A270
person.identifier.ciencia-idE31F-1630-42BB
person.identifier.orcid0000-0003-2462-9930
person.identifier.orcid0000-0003-4237-8952
person.identifier.orcid0000-0001-9936-7336
person.identifier.ridK-9199-2013
person.identifier.scopus-author-id23486193000
person.identifier.scopus-author-id7005174717
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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