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In vitro metabolism of steroid hormones by ovary and hepatopancreas of the crustacean Penaeid shrimp Marsupenaeus japonicus
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Autores
Monteiro, Pedro
Summavielle, Teresa
Orientador(es)
Resumo(s)
Radioimmunoassay (RIA) was applied to identify selected metabolites obtained in in vitroincubations of ovarianand hepatopancreatic tissues from female Marsupenaeus japonicusin previtellogenic and vitellogenic maturation stages. Depend-ing on the precursors used, progesterone, 17α-hydroxyprogesterone or testosterone, it was possible to observe the presence of sev-eral enzymatic activities through metabolites detected in incubations. The present study provides evidence that the ovary of theshrimpM. japonicusis capable of synthesising 17β-estradiol from progesterone, evidencing the presence of 17α-hydroxy-lase, C17-C20lyase, 17β-hydroxysteroid dehydrogenase (17β-HSD) and aromatase. Enzymatic activities of aromatase, 3β-HSD and 17α-hydroxylase were detected in hepatopancreas, but not that of C17-C20lyase. These results suggest that 17β-estradiol could be the vitellogenesis stimulating ovarian hormone (VSOH), and together with former in vivoand in vitrostudies this leads us to propose that in fact there may be a physiological role for steroid hormones in the maturation cycleof crustaceans.
Descrição
Palavras-chave
Crustacean Decapods Hepatopancreas Hormones Maturation Ovary Reproduction Steroids
Contexto Educativo
Citação
Summavielle, T., Monteiro, P. R. R., Reis-Henriques, M. A., & Coimbra, J. (2003). In vitro metabolism of steroid hormones by ovary and hepatopancreas of the crustacean Penaeid shrimp Marsupenaeus japonicus. Scientia Marina, 67(3), Artigo 3. https://doi.org/10.3989/scimar.2003.67n3299
Editora
Editorial CSIC