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Simultaneous distillation–extraction of high-value volatile compounds from Cistus ladanifer L.
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Orientador(es)
Resumo(s)
The present paper describes a procedure to isolate volatiles from rock-rose (Cistus ladanifer L.) using simultaneous distillation–extraction (SDE).
High-value volatile compounds (HVVC) were selected and the influence of the extraction conditions investigated. The effect of the solvent nature
and extraction time on SDE efficiency was studied. The best performance was achieved with pentane in 1 h operation. The extraction efficiencies
ranged from 65% to 85% and the repeatability varied between 4% and 6% (as a CV%).
The C. ladanifer SDE extracts were analysed by headspace solid phase microextraction (HS-SPME) followed by gas chromatography with
flame ionization detection (GC-FID). The HS-SPME sampling conditions such as fiber coating, temperature, ionic strength and exposure time were
optimized. The best results were achieved with an 85 µm polyacrylate fiber for a 60 min headspace extraction at 40ºC with 20% (w/v) of NaCl.
For optimized conditions the recovery was in average higher than 90% for all compounds and the intermediate precision ranged from 4 to 9% (as
CV %). The volatiles α-pinene (22.2 mg g−1 of extract), 2,2,6-trimethylcyclohexanone (6.1 mg g−1 of extract), borneol (3.0 mg g−1 of extract) and
bornyl acetate (3.9 mg g−1 of extract) were identified in the SDE extracts obtained from the fresh plant material.
Descrição
Palavras-chave
Simultaneous distillation–extraction Solid phase microextraction Cistus ladanifer L. Volatiles
Contexto Educativo
Citação
Editora
Elsevier