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DNA-based biosensor for the electrocatalytic determination of antioxidant capacity in beverages

2011Journal article Open access
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dc.contributor.authorBarroso, M. Fátima
dc.contributor.authorde-los-Santos-Álvarez, N.
dc.contributor.authorLobo-Castañón, M. J.
dc.contributor.authorMiranda-Ordieres, A. J.
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorOliveira, M. Beatriz P. P.
dc.contributor.authorTuñón-Blanco, P.
dc.date.accessioned2013-11-27T12:12:16Z
dc.date.available2013-11-27T12:12:16Z
dc.date.issued2011
dc.description.abstractReactive oxygen species (ROS) are produced as a consequence of normal aerobic metabolism and are able to induce DNA oxidative damage. At the cellular level, the evaluation of the protective effect of antioxidants can be achieved by examining the integrity of the DNA nucleobases using electrochemical techniques. Herein, the use of an adenine-rich oligonucleotide (dA21) adsorbed on carbon paste electrodes for the assessment of the antioxidant capacity is proposed. The method was based on the partial damage of a DNA layer adsorbed on the electrode surface by OH• radicals generated by Fenton reaction and the subsequent electrochemical oxidation of the intact adenine bases to generate an oxidation product that was able to catalyze the oxidation of NADH. The presence of antioxidant compounds scavenged hydroxyl radicals leaving more adenines unoxidized, and thus, increasing the electrocatalytic current of NADHmeasured by differential pulse voltammetry (DPV). Using ascorbic acid (AA) as a model antioxidant species, the detection of as low as 50nMof AA in aqueous solution was possible. The protection efficiency was evaluated for several antioxidant compounds. The biosensor was applied to the determination of the total antioxidant capacity (TAC) in beverages.por
dc.identifier10.1016/j.bios.2010.10.019
dc.identifier.doi10.1016/j.bios.2010.10.019pt_PT
dc.identifier.issn0956-5663
dc.identifier.urihttp://hdl.handle.net/10400.22/2984
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.ispartofseriesBiosensors and Bioelectronics; Vol. 26, Issue 5
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0956566310007049por
dc.subjectNADHpor
dc.subjectDNA biosensorpor
dc.subjectElectrocatalytic oxidationpor
dc.subjectTotal antioxidant capacity (TAC)por
dc.subjectAscorbic acidpor
dc.subjectReactive oxygen species (ROS)por
dc.titleDNA-based biosensor for the electrocatalytic determination of antioxidant capacity in beveragespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage2401por
oaire.citation.startPage2396por
person.familyNamede Sá Barroso
person.familyNameDelerue-Matos
person.givenNameMaria de Fátima
person.givenNameCristina
person.identifier.ciencia-idAF16-22E5-5EBD
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0001-9011-5992
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id25631648000
person.identifier.scopus-author-id6603741848
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication3d701c85-4580-4614-be6d-5a1abc8cf729
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication.latestForDiscovery3d701c85-4580-4614-be6d-5a1abc8cf729

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