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Comprehensive Analysis of Secreted Protein, Acidic and Rich in Cysteine in Prostate Carcinogenesis: Development of a 3D Nanostructured Bone-Like Model

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dc.contributor.authorRibeiro, Nilza
dc.contributor.authorCosta-Pinheiro, Pedro
dc.contributor.authorHenrique, Rui
dc.contributor.authorGomez-Lazaro, Maria
dc.contributor.authorPereira, Marisa P.
dc.contributor.authorMansur, Alexandra A. P.
dc.contributor.authorMansur, Herman S.
dc.contributor.authorJerónimo, Carmen
dc.contributor.authorSousa, Susana R.
dc.contributor.authorMonteiro, Fernando J.
dc.date.accessioned2017-01-11T15:54:16Z
dc.date.embargo2116
dc.date.issued2016
dc.description.abstractMost aggressive prostate cancer (PCa) types tend to metastasize frequently to bone and SPARC, a matricellular protein, might participate in such biological processes. The objective of this study was to evaluate the effect of SPARC in prostate carcinogenesis and bone metastization. This was explored assessing the morphology, metabolic activity and SPARC expression of different PCa cell lines resembling different stages of carcinogenesis, using a 3D bone-biomimetic model (collagen nanofibers/nanohydroxyapatite) grafted with SPARC. Our findings highlight distinct cellular behavior depending on cell type and presence of exogenous SPARC. In fact, SPARC addition contributed to the survival and significant growth of a non-bone metastatic PCa cell line (LNCaP) on bone-like biomaterial. Moreover, SPARC expression levels were evaluated in a series of prostatic tissues, comparing normal prostate, pre-neoplastic prostate intraepithelial neoplasias and overtly malignant tumors, and also metastasis to its correspondent primary prostate tumors, ascertaining potential association between SPARC and clinicopathological data. Remarkably, SPARC was overexpressed in patients with higher Gleason Score, indicating tumors with poor prognosis, as well as in metastasis, particularly from bone sites, compared with their respective primary tumors. The results suggest a potential role of SPARC as a clinical target on PCa, due to its association with bone metastization.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1166/jbn.2016.2276pt_PT
dc.identifier.issn1550-7033
dc.identifier.urihttp://hdl.handle.net/10400.22/9228
dc.language.isoengpt_PT
dc.publisherAmerican Scientific Publisherspt_PT
dc.relation.ispartofseriesJournal of Biomedical Nanotechnology;Vol. 12, nº 8
dc.relation.publisherversionhttp://www.ingentaconnect.com/content/asp/jbn/2016/00000012/00000008/art00007pt_PT
dc.subject3D bone-biomimetic biomaterialpt_PT
dc.subjectBone metastasispt_PT
dc.subjectGleason scorept_PT
dc.subjectProstate cancerpt_PT
dc.subjectQuantum dotspt_PT
dc.subjectSPARCpt_PT
dc.titleComprehensive Analysis of Secreted Protein, Acidic and Rich in Cysteine in Prostate Carcinogenesis: Development of a 3D Nanostructured Bone-Like Modelpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1678pt_PT
oaire.citation.startPage1667pt_PT
oaire.citation.titleJournal of Biomedical Nanotechnologypt_PT
person.familyNameR. Sousa
person.givenNameSusana
person.identifier820351
person.identifier.ciencia-id2D13-F15F-D519
person.identifier.orcid0000-0001-8018-4310
person.identifier.ridD-5356-2013
person.identifier.scopus-author-id10045389000
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication13159acc-5117-4c06-9ee1-02820b9b6218
relation.isAuthorOfPublication.latestForDiscovery13159acc-5117-4c06-9ee1-02820b9b6218

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