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Diamine oxidase-modified screen-printed electrode for the redox-mediated determination of histamine

2020Journal article Open access
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dc.contributor.authorTorre, Ricarda
dc.contributor.authorCosta Rama, Estefanía
dc.contributor.authorNouws, Henri
dc.contributor.authorDelerue-Matos, Cristina
dc.date.accessioned2021-09-27T11:06:57Z
dc.date.available2021-09-27T11:06:57Z
dc.date.issued2020
dc.description.abstractHistamine is an important biogenic amine because of its role in immune responses and the regulation of physiological functions. It is also used as a food freshness indicator, so its maximum concentration in fish is legally regulated. Although several robust and sensitive methods for histamine detection are already available, it continues to be a challenge to develop simple and portable devices that allow rapid histamine screening at any point of the fish production chain. Thus, in this work, a simple, miniaturized and low-cost sensor for histamine analysis was developed. The construction of the sensor only takes 30 min and consists of the immobilization of the enzyme diamine oxidase on the surface of a screen-printed carbon electrode by cross-linking. The quantification of histamine was achieved by chronoamperometry (+ 0.2V,120 s) using hexacyanoferrate (III) as a redox mediator. This selective sensor provided a low limit of detection (0.97 mg L−1) and accurate and precise results and was successfully applied to the analysis of spiked tuna and mackerel extracts,obtaining recovery values of 99–100%. Moreover, the sensor shows good stability, maintaining 87.7% of its initial signalafter 35 days.pt_PT
dc.description.sponsorshipThis work was supported by UID/QUI/50006/2019 with funding from the Fundação para a Ciência e a Tecnologia (FCT)/the Ministério da Ciência, Tecnologia e Ensino Superior (MCTES) through national funds. The authors also thank FCT and the EU for funding through the projects: FOODnanoHEALTH - Qualidade e Segurança Alimentar –uma abordagem (nano)tecnológica (Portugal2020, Norte-01-0145-FEDER-000011), FishBioSensing - Portable electrochemical (bio)sensing devices for safety and quality assessment of fishery products (02/SAICT/2016, POCI-01-0145-FEDER-023817), PTDC/ASP-PES/29547/2017 –CECs(Bio)Sensing - (Bio)sensors for assessment of contaminants of emerging concern in fishery commodities, (POCI-01-0145-FEDER-029547) and PTDC/QUI-QAN/30735/2017 –TracAllerSens - Electrochemical sensors for the detection and quantification of trace amounts of allergens in food products (POCI-01-0145-FEDER-030735), supported by national funds by FCT / MCTES and co-supported by Fundo Europeu de esenvolvimento Regional (FEDER) through COMPETE 2020 –Programa Operacional Competitividade e Internacionalização. E. Costa-Rama thanks the Government of Principado de Asturias and Marie Curie-Cofund Actions for the post-doctoral grant “Clarín-Cofund”ACA17-20. R. Torre is grateful for her PhD grant from FCT (SFRH/BD/143753/2019), fi- nanced by POPH–QREN–Tipologia 4.1–Formação Avançada, and subsidized by FSE and MCTESpt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1186/s40543-020-0203-3pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/18560
dc.language.isoengpt_PT
dc.publisherSpringerpt_PT
dc.relation.publisherversionhttps://jast-journal.springeropen.com/articles/10.1186/s40543-020-0203-3pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectAmperometric biosensorpt_PT
dc.subjectEnzymatic sensorpt_PT
dc.subjectBiogenic aminept_PT
dc.subjectHistaminept_PT
dc.subjectDiamine oxidasept_PT
dc.subjectRedox mediatorpt_PT
dc.subjectFerrocyanidept_PT
dc.subjectScreen-printed electrodept_PT
dc.titleDiamine oxidase-modified screen-printed electrode for the redox-mediated determination of histaminept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/154751/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/150733/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/151929/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/74433/PT
oaire.citation.issue1pt_PT
oaire.citation.titleJournal of Analytical Science and Technologypt_PT
oaire.citation.volume11pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream9471 - RIDTI
oaire.fundingStream9471 - RIDTI
oaire.fundingStreamPOR_NORTE
person.familyNameTorre
person.familyNameCosta Rama
person.familyNameNouws
person.familyNameDelerue-Matos
person.givenNameRicarda
person.givenNameEstefanía
person.givenNameHendrikus
person.givenNameCristina
person.identifier1046746
person.identifier.ciencia-id2015-9883-AD97
person.identifier.ciencia-id3614-E403-0136
person.identifier.ciencia-id9B1A-9CED-6EE3
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0003-2843-8402
person.identifier.orcid0000-0002-5357-9269
person.identifier.orcid0000-0002-1472-8866
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridJ-5815-2018
person.identifier.ridD-5249-2013
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id36703129800
person.identifier.scopus-author-id7801450247
person.identifier.scopus-author-id6603741848
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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