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Lab-on-valve automated and miniaturized assessment of nanoparticle concentration based on light-scattering

2023-02-21Journal article Restricted access
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dc.contributor.authorMarques, Sara S.
dc.contributor.authorRamos, Inês I.
dc.contributor.authorSilva, Carla
dc.contributor.authorBarreiros, Luisa
dc.contributor.authorDomingues, Maria R.
dc.contributor.authorSegundo, Marcela A.
dc.date.accessioned2023-04-12T15:20:06Z
dc.date.available2023-04-12T15:20:06Z
dc.date.issued2023-02-21
dc.description.abstractNanoparticles (NPs) concentration directly impacts the dose delivered to target tissues by nanocarriers. The evaluation of this parameter is required during NPs developmental and quality control stages, for setting dose−response correlations and for evaluating the reproduci bility of the manufacturing process. Still, faster and simpler procedures, dismissing skilled operators and post-analysis conversions are needed to quantify NPs for research and quality control operations, and to support result validation. Herein, a miniaturized automated ensemble method to measure NPs concentration was established under the lab-on-valve (LOV) mesofluidic platform. Automatic NPs sampling and delivery to the LOV detection unit were set by flow programming. NPs concentration measurements were based on the decrease in the light transmitted to the detector due to the light scattered by NPs when passing through the optical path. Each analysis was accomplished in 2 min, rendering a determination throughput of 30 h−1 (6 samples h−1 for n = 5) and only requiring 30 μL (≈0.03 g) of NPs suspension. Measurements were performed on polymeric NPs, as these represent one of the major classes of NPs under development for drug delivery aims. Determinations for polystyrene NPs (of 100, 200, and 500 nm) and for NPs made of PEGylated poly-D,L-lactide-co glycolide (PEG−PLGA, a biocompatible FDA-approved polymer) were accomplished within 108 −1012 particles mL−1 range, depending on the NPs size and composition. NPs size and concentration were maintained during analysis, as verified for NPs eluted from the LOV by particle tracking analysis (PTA). Moreover, concentration measurements for PEG−PLGA NPs loaded with an anti-inflammatory drug, methotrexate (MTX), after their incubation in simulated gastric and intestinal fluids were successfully achieved (recovery values of 102−115%, as confirmed by PTA), showing the suitability of the proposed method to support the development of polymeric NPs targeting intestinal delivery.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMarques, S. S., Ramos, I. I., Silva, C., Barreiros, L., Domingues, M. R., & Segundo, M. A. (2023). Lab-on-Valve Automated and Miniaturized Assessment of Nanoparticle Concentration Based on Light-Scattering. Analytical Chemistry, 95(10), 4619–4626. https://doi.org/10.1021/acs.analchem.2c04631pt_PT
dc.identifier.doi10.1021/acs.analchem.2c04631pt_PT
dc.identifier.eissn1520-6882
dc.identifier.issn0003-2700
dc.identifier.urihttp://hdl.handle.net/10400.22/22719
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherACS Publicationspt_PT
dc.relation.publisherversionhttps://pubs.acs.org/doi/10.1021/acs.analchem.2c04631pt_PT
dc.subjectAromatic compoundspt_PT
dc.subjectFluidspt_PT
dc.subjectHydrocarbonspt_PT
dc.subjectLightpt_PT
dc.subjectNanoparticlespt_PT
dc.titleLab-on-valve automated and miniaturized assessment of nanoparticle concentration based on light-scatteringpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage4626pt_PT
oaire.citation.startPage4619pt_PT
oaire.citation.titleAnalytical Chemistrypt_PT
oaire.citation.volume95 (10)pt_PT
person.familyNameBarreiros
person.givenNameLuisa
person.identifier.ciencia-id611F-E0C5-0230
person.identifier.orcid0000-0003-3481-5809
person.identifier.ridD-7950-2013
person.identifier.scopus-author-id6508205485
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication1e66bacc-64de-4ecb-96b7-4c0e366cba57
relation.isAuthorOfPublication.latestForDiscovery1e66bacc-64de-4ecb-96b7-4c0e366cba57

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