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Molecularly imprinted electrochemical sensor for ochratoxin A detection in food samples

2015-08Journal article Restricted access
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dc.contributor.authorPacheco, João G.
dc.contributor.authorCastro, Mafalda
dc.contributor.authorMachado, Susana
dc.contributor.authorBarroso, M. Fátima
dc.contributor.authorNouw, Henri P.A.
dc.contributor.authorDelerue-Matos, Cristina
dc.date.accessioned2015-12-22T15:26:14Z
dc.date.available2015-12-22T15:26:14Z
dc.date.issued2015-08
dc.description.abstractA novel electrochemical sensor for ochratoxin A (OTA) detection was fabricated through the modification of a glassy carbon electrode (GCE) with multiwalled carbon nanotubes (MWCNTs) and a molecularly imprinted polymer (MIP). The MWCNTs dramatically promoted the sensitivity of the developed sensor, while polypyrrole (PPy) imprinted with OTA served as the selective recognition element. The imprinted PPy film was prepared by electropolymerization of pyrrole in the presence of OTA as a template molecule via cyclic voltammetry (CV). The electrochemical oxidation of OTA at the developed sensor was investigated by CV and differential pulse voltammetry (DPV). The developed MIP/MWCNT/GCE sensor showed a linear relationship, when using DPV, between peak current intensity and OTA concentration in the range between 0.050 and 1.0 μM, with limits of detection (LOD) and quantification of 0.0041 μM (1.7 μg/L) and 0.014 μM (5.7 μg/L) respectively. With the developed sensor precise results were obtained; relative standard deviations of 4.2% and 7.5% in the evaluation of the repeatability and reproducibility, respectively. The MIP/MWCNT/GCE sensor is simple to fabricate and easy to use and was successfully applied to the determination of OTA in spiked beer and wine samples, with recoveries between 84 and 104%, without the need of a sample pre-treatment step.pt_PT
dc.identifier.doi10.1016/j.snb.2015.03.046pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/7221
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.ispartofseriesSensors and Actuators B: Chemical;Vol. 215
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0925400515003706pt_PT
dc.subjectOchratoxin Apt_PT
dc.subjectElectrochemical sensorpt_PT
dc.subjectMolecularly imprinted polymerpt_PT
dc.subjectMultiwalled carbon nanotubespt_PT
dc.titleMolecularly imprinted electrochemical sensor for ochratoxin A detection in food samplespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage112pt_PT
oaire.citation.startPage107pt_PT
oaire.citation.titleSensors and Actuators B: Chemicalpt_PT
oaire.citation.volume215pt_PT
person.familyNamede Sá Barroso
person.familyNameDelerue-Matos
person.givenNameMaria de Fátima
person.givenNameCristina
person.identifier.ciencia-idAF16-22E5-5EBD
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0001-9011-5992
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id25631648000
person.identifier.scopus-author-id6603741848
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication3d701c85-4580-4614-be6d-5a1abc8cf729
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication.latestForDiscovery3d701c85-4580-4614-be6d-5a1abc8cf729

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