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Osteoclastogenic differentiation of human precursor cells over micro- and nanostructured hydroxyapatite topography

2016Journal article Restricted access
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dc.contributor.authorCosta-Rodrigues, Joao
dc.contributor.authorCarmo, S.
dc.contributor.authorPerpétuo, I. P.
dc.contributor.authorMonteiro, F. J.
dc.contributor.authorFernandes, M. H.
dc.date.accessioned2017-07-13T11:27:44Z
dc.date.embargo2117
dc.date.issued2016
dc.description.abstractBackground: Surface topography is a key parameter in bone cells–biomaterials interactions. This study analyzed the behavior of human osteoclast precursor cells cultured over three hydroxyapatite (HA) surfaces ranging from a micro- to nanoscale topography. Methods: HA surfaces were prepared with microsized HA particles, at 1300 °C (HA1), andwith nanosized HA particles at 1000 °C (HA2) and 830 °C (HA3). Human osteoclast precursorswere cultured in the absence or presence of M-SCF and RANKL. Results: HA surfaces had similar chemical composition, however, HA1 and HA3 presented typical micro- and nanostructured topographies, respectively, and HA2 profile was between those of HA1 and HA3. The decrease on the average grain diameter to the nanoscale range (HA3)was accompanied by an increase in surface area, porosity and hydrophilicity and a decrease in roughness. Compared to HA1 surface, HA3 allowed a lower osteoclastic adhesion, differentiation and function. Differences in the cell response appeared to be associated with the modulation of relevant intracellular signaling pathways. Conclusions: The decrease in HA grain size to a biomimetic nanoscale range, appears less attractive to osteoclastic differentiation and function, compared to the HA microsized topography. General significance: This observation emphasizes the role of surface topography in designing advanced biomaterials for tailored bone cells response in regenerative strategies.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCosta-Rodrigues, J., Carmo, S., Perpétuo, I. P., Monteiro, F. J., & Fernandes, M. H. (2016). Osteoclastogenic differentiation of human precursor cells over micro- and nanostructured hydroxyapatite topography. Biochimica et Biophysica Acta (BBA) - General Subjects, 1860(4), 825–835. https://doi.org/10.1016/j.bbagen.2016.01.014
dc.identifier.doi10.1016/j.bbagen.2016.01.014pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/10044
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relation.ispartofseriesBiochimica et Biophysica Acta (BBA) - General Subjects;Vol. 1860, Issue 4
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0304416516000234pt_PT
dc.subjectOsteoclastogenesispt_PT
dc.subjectHydroxyapatitept_PT
dc.subjectMicrotopographypt_PT
dc.subjectNanotopographypt_PT
dc.titleOsteoclastogenic differentiation of human precursor cells over micro- and nanostructured hydroxyapatite topographypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage835pt_PT
oaire.citation.startPage825pt_PT
oaire.citation.titleBBA - Biochimica et Biophysica Actapt_PT
person.familyNameCosta-Rodrigues
person.givenNameJoao
person.identifier.ciencia-id7412-B006-2120
person.identifier.orcid0000-0003-1375-8067
person.identifier.scopus-author-id8558383400
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationce85cc8f-09f3-4c26-aeaf-6128971acf68
relation.isAuthorOfPublication.latestForDiscoveryce85cc8f-09f3-4c26-aeaf-6128971acf68

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