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Enzymatic determination of choline in milk using a FIA system with potentiometric detection
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The development of a FIA system for the determination of total choline content in several types of milk is
described. The samples were submitted to hydrochloric acid digestion before injection into the system and passed
through an enzymatic reactor containing choline oxidase immobilised on glass beads. This enzymatic reaction
releases hydrogen peroxide which then reacts with a solution of iodide. The decrease in the concentration of
iodide ion is quantified using an iodide ion selective tubular electrode based on a homogeneous crystalline
membrane. Validation of the results obtained with this system was performed by comparison with results from a
method described in the literature and applied to the determination of total choline in milks. The relative deviation
was always < 5%. The repeatability of the method developed was assessed by calculation of the relative standard
deviation (RSD) for 12 consecutive injections of one sample. The RSD obtained was < 0.6%.
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Royal Society of Chemistry