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High-performance electrochemical immunomagnetic assay for breast cancer analysis

2020Journal article Open access
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dc.contributor.authorFreitas, Maria
dc.contributor.authorNouws, Henri P. A.
dc.contributor.authorKeating, Elisa
dc.contributor.authorDelerue-Matos, Cristina
dc.date.accessioned2022-01-13T14:33:23Z
dc.date.available2022-01-13T14:33:23Z
dc.date.issued2020
dc.description.abstractDespite the evolution of targeted therapies in oncology, some challenges such as screening and early diagnosis of cancer-related biomarkers still remain. The analysis of the Human Epidermal growth factor Receptor 2 (HER2) in biological fluids provides essential information for effective treatments. In this work we report the development of an electrochemical immunomagnetic bioassay for the analysis of the extracellular domain of HER2 (HER2-ECD) in human serum and cancer cells. Biomodified carboxylic acid functionalized magnetic beads (COOH-MBs) were used as the capture probe and an antibody labelled with alkaline phosphatase (AP) as the signalling probe. In the presence of HER2-ECD a sandwich complex was formed on the MBs, which were magnetically attracted to the surface of a screen-printed carbon electrode (SPCE). After the addition of 3-indoxyl phosphate and silver ions, used as the enzymatic substrate, the immunological interaction was detected by linear sweep voltammetry. Two linear concentration ranges were established: one between 5.0 and 50 ng/mL and another between 50 and 100 ng/mL. The developed assay provided a clinically useful detection limit (2.8 ng/mL) and has an adequate precision (Vx0 < 5%). The assay provided accurate results and was selective towards the target biomarker. Additionally, CTCs were analysed in human serum and a detection limit of 3 cells/mL was achieved for the HER+ breast cancer cell line SK-BR-3.pt_PT
dc.description.sponsorshipThe authors are grateful for the financial support from the Fundação para a Ciência e a Tecnologia (FCT) / the Ministério da Ciência, Tecnologia e Ensino Superior (MCTES) through national funds (Portugal) (LAQV - UID/QUI/50006/2019 and CINTESIS - UID/IC/4255/2019). Maria Freitas is grateful to FCT for her PhD grant (SFRH/BD/111942/2015), financed by POPH- QREN-Tipologia 4.1-Formação Avançada, subsidized by Fundo Social Europeu and the MCTES. The authors are also thankful to Rui Rocha and CEMUP “Centro de Materiais da Universidade do Porto” for the SEM workpt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFreitas M, Nouws HPA, Keating E, Delerue-Matos C, High-performance electrochemical immunomagnetic assay for breast cancer analysis, Sensors and Actuators: B. Chemical (2020), doi: https://doi.org/10.1016/j.snb.2020.127667pt_PT
dc.identifier.doi10.1016/j.snb.2020.127667pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/19453
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationHigh-performance magnetic nanoparticle-based immunoassays for breast cancer biomarker analysis
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/abs/pii/S0925400520300149pt_PT
dc.subjectBreast cancerpt_PT
dc.subjectHER2-ECDpt_PT
dc.subjectSK-BR-3pt_PT
dc.subjectElectrochemical immunoassaypt_PT
dc.subjectMagnetic beadspt_PT
dc.subjectScreen printed electrodespt_PT
dc.titleHigh-performance electrochemical immunomagnetic assay for breast cancer analysispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHigh-performance magnetic nanoparticle-based immunoassays for breast cancer biomarker analysis
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FIC%2F04255%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F111942%2F2015/PT
oaire.citation.startPage127667pt_PT
oaire.citation.titleSensors and Actuators B: Chemicalpt_PT
oaire.citation.volume308pt_PT
oaire.fundingStream5876
oaire.fundingStream5876
oaire.fundingStreamOE
person.familyNameNouws
person.familyNameDelerue-Matos
person.givenNameHendrikus
person.givenNameCristina
person.identifier1046746
person.identifier.ciencia-idBC12-319B-037F
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person.identifier.orcid0000-0002-0628-2753
person.identifier.orcid0000-0002-1472-8866
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridD-5249-2013
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id7801450247
person.identifier.scopus-author-id6603741848
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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