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Molinate quantification in environmental water by a glutathione-S-transferase based biosensor

2013Journal article Open access
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dc.contributor.authorOliveira, Túlio I. S.
dc.contributor.authorOliveira, Marcela
dc.contributor.authorViswanathan, Subramanian
dc.contributor.authorBarroso, M. Fátima
dc.contributor.authorBarreiros, Luísa
dc.contributor.authorNunes, O. C.
dc.contributor.authorRodrigues, José A.
dc.contributor.authorLima-Neto, Pedro de
dc.contributor.authorMazzetto, Selma E.
dc.contributor.authorMorais, Simone
dc.contributor.authorDelerue-Matos, Cristina
dc.date.accessioned2014-01-17T12:56:29Z
dc.date.available2014-01-17T12:56:29Z
dc.date.issued2013
dc.description.abstractA glutathione-S-transferase (GST)based biosensor was developed to quantify the thiocarbamate herbicide molinate in environmental water.The biosensor construction was based on GST immobilization onto a glassy carbon electrode via aminosilane–glutaraldehyde covalent attachment. The principle supporting the use of this biosensor consists of the GST inhibition process promoted by molinate. Differential pulse voltammetry was used to obtain a calibration curve for molinate concentration, ranging from 0.19 to 7.9 mgL -1 and presenting a detection limit of 0.064 mgL- 1. The developed biosensor is stable,and reusable during 15 days.The GST-based biosensor was successfully applied to quantify molinate in rice paddy field floodwater samples. The results achieved with the developed biosensor were in accordance with those obtained by high performance liquid chromatography. The proposed device is suitable for screening environmental water analysis and, since no sample preparation is required, it can be used in situ and in real-time measurements.por
dc.identifier.doi10.1016/j.talanta.2012.10.074pt_PT
dc.identifier.issn0039-9140
dc.identifier.urihttp://hdl.handle.net/10400.22/3346
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.ispartofseriesTalanta; Vol. 106
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0039914012009058#por
dc.subjectGlutathione-S-transferasepor
dc.subjectEnzymatic biosensorpor
dc.subjectVoltammetrypor
dc.subjectMolinatepor
dc.subjectRice paddy field floodwaterpor
dc.titleMolinate quantification in environmental water by a glutathione-S-transferase based biosensorpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage254por
oaire.citation.startPage249por
oaire.citation.titleTalantapor
oaire.citation.volume106por
person.familyNamede Sá Barroso
person.familyNameMorais
person.familyNameDelerue-Matos
person.givenNameMaria de Fátima
person.givenNameSimone
person.givenNameCristina
person.identifier1598822
person.identifier.ciencia-idAF16-22E5-5EBD
person.identifier.ciencia-idB111-BBFB-F200
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0001-9011-5992
person.identifier.orcid0000-0001-6433-5801
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id25631648000
person.identifier.scopus-author-id7007053747
person.identifier.scopus-author-id6603741848
rcaap.rightsclosedAccesspor
rcaap.typearticlepor
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relation.isAuthorOfPublication7a9c7d63-a6c7-4159-b12d-b049b8c5c8f9
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication.latestForDiscovery09f6a7bd-2f15-42b0-adc5-04bd22210519

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