Publication
Electroanalysis of urinary L-dopa using tyrosinase immobilized on gold nanoelectrode ensembles
| dc.contributor.author | Pinho, Ana | |
| dc.contributor.author | Viswanathan, Subramanian | |
| dc.contributor.author | Ribeiro, Susana | |
| dc.contributor.author | Oliveira, M. Beatriz P. P. | |
| dc.contributor.author | Delerue-Matos, Cristina | |
| dc.date.accessioned | 2013-09-27T10:54:35Z | |
| dc.date.available | 2013-09-27T10:54:35Z | |
| dc.date.issued | 2012 | |
| dc.description.abstract | The performance of an amperometric biosensor constructed by associating tyrosinase (Tyr) enzyme with the advantages of a 3D gold nanoelectrode ensemble (GNEE) is evaluated in a flow-injection analysis (FIA) system for the analysis of l-dopa. GNEEs were fabricated by electroless deposition of the metal within the pores of polycarbonate track-etched membranes. A simple solvent etching procedure based on the solubility of polycarbonate membranes is adopted for the fabrication of the 3D GNEE. Afterward, enzyme was immobilized onto preformed self-assembled monolayers of cysteamine on the 3D GNEEs (GNEE-Tyr) via cross-linking with glutaraldehyde. The experimental conditions of the FIA system, such as the detection potential (−0.200 V vs. Ag/AgCl) and flow rates (1.0 mL min−1) were optimized. Analytical responses for l-dopa were obtained in a wide concentration range between 1 × 10−8 mol L−1 and 1 × 10−2 mol L−1. The limit of quantification was found to be 1 × 10−8 mol L−1 with a resultant % RSD of 7.23% (n = 5). The limit of detection was found to be 1 × 10−9 mol L−1 (S/N = 3). The common interfering compounds, namely glucose (10 mmol L−1), ascorbic acid (10 mmol L−1), and urea (10 mmol L−1), were studied. The recovery of l-dopa (1 × 10−7 mol L−1) from spiked urine samples was found to be 96%. Therefore, the developed method is adequate to be applied in the clinical analysis. | por |
| dc.identifier.doi | 10.1007/s10800-012-0379-3 | pt_PT |
| dc.identifier.issn | 0021-891X | |
| dc.identifier.uri | http://hdl.handle.net/10400.22/2019 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Springer Netherlands | por |
| dc.relation.ispartofseries | Journal of Applied Electrochemistry; Vol.42, Issue 3 | |
| dc.relation.publisherversion | http://link.springer.com/article/10.1007%2Fs10800-012-0379-3 | por |
| dc.subject | Gold nanoelectrode | por |
| dc.subject | Tyrosinase | por |
| dc.subject | L-dopa | por |
| dc.subject | Electrochemical biosensor | por |
| dc.title | Electroanalysis of urinary L-dopa using tyrosinase immobilized on gold nanoelectrode ensembles | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 137 | por |
| oaire.citation.startPage | 131 | por |
| oaire.citation.volume | 42 | por |
| person.familyName | Delerue-Matos | |
| person.givenName | Cristina | |
| person.identifier.ciencia-id | 9A1A-43FB-5C27 | |
| person.identifier.orcid | 0000-0002-3924-776X | |
| person.identifier.rid | D-4990-2013 | |
| person.identifier.scopus-author-id | 6603741848 | |
| rcaap.rights | openAccess | por |
| rcaap.type | article | por |
| relation.isAuthorOfPublication | 09f6a7bd-2f15-42b0-adc5-04bd22210519 | |
| relation.isAuthorOfPublication.latestForDiscovery | 09f6a7bd-2f15-42b0-adc5-04bd22210519 |