Publication
Optimization of QuEChERS procedure coupled to GC-ECD for organochlorine pesticide determination in carrot samples
| dc.contributor.author | Correia-Sá, Luísa | |
| dc.contributor.author | Fernandes, Virgínia C. | |
| dc.contributor.author | Calhau, Conceição | |
| dc.contributor.author | Domingues, Valentina F. | |
| dc.contributor.author | Delerue-Matos, Cristina | |
| dc.date.accessioned | 2014-01-22T11:44:50Z | |
| dc.date.available | 2014-01-22T11:44:50Z | |
| dc.date.issued | 2013 | |
| dc.description.abstract | An optimised version of the Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) method for simultaneous determination of 14 organochlorine pesticides in carrots was developed using gas chromatography coupled with electron-capture detector (GC-ECD) and confirmation by gas chromatography tandem mass spectrometry (GC-MS/MS). A citrate-buffered version of QuEChERS was applied for the extraction of the organochlorine pesticides, and for the extract clean-up, primary secondary amine, octadecyl-bonded silica (C18), magnesium sulphate (MgSO4) and graphitized carbon black were used as sorbents. The GC-ECD determination of the target compounds was achieved in less than 20 min. The limits of detection were below the EUmaximum residue limits (MRLs) for carrots, 10–50 μg kg−1, while the limit of quantification did exceed 10 μg kg−1 for hexachlorobenzene (HCB). The introduction of a sonication step was shown to improve the recoveries. The overall average recoveries in carrots, at the four tested levels (60, 80, 100 and 140 μg kg−1), ranged from 66 to 111% with relative standard deviations in the range of 2– 15 % (n03) for all analytes, with the exception of HCB. The method has been applied to the analysis of 21 carrot samples from different Portuguese regions, and β-HCH was the pesticide most frequently found, with concentrations oscillating between less than the limit of quantification to 14.6 μg kg−1. Only one sample had a pesticide residue (β-HCH) above the MRL, 14.6 μg kg−1. This methodology combines the advantages of both QuEChERS and GC-ECD, producing a very rapid, sensitive and reliable procedure which can be applied in routine analytical laboratories. | por |
| dc.identifier.doi | 10.1007/s12161-012-9463-x | pt_PT |
| dc.identifier.issn | 1936-976X | |
| dc.identifier.issn | 1936-9751 | |
| dc.identifier.uri | http://hdl.handle.net/10400.22/3432 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Springer | por |
| dc.relation.ispartofseries | Food Analytical Methods; Vol. 6, Issue 2 | |
| dc.relation.publisherversion | http://link.springer.com/article/10.1007/s12161-012-9463-x | por |
| dc.subject | QuEChERS | por |
| dc.subject | Carrots | por |
| dc.subject | Organochlorine pesticides | por |
| dc.subject | GC-ECD | por |
| dc.subject | Tandem mass spectrometry | por |
| dc.title | Optimization of QuEChERS procedure coupled to GC-ECD for organochlorine pesticide determination in carrot samples | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 597 | por |
| oaire.citation.issue | Issue 2 | |
| oaire.citation.startPage | 587 | por |
| oaire.citation.title | Food Analytical Methods | por |
| oaire.citation.volume | Vol. 6 | por |
| person.familyName | Domingues | |
| person.familyName | Delerue-Matos | |
| person.givenName | Valentina Maria Fernandes | |
| person.givenName | Cristina | |
| person.identifier.ciencia-id | 4E16-791D-6664 | |
| person.identifier.ciencia-id | 9A1A-43FB-5C27 | |
| person.identifier.orcid | 0000-0003-3472-849X | |
| person.identifier.orcid | 0000-0002-3924-776X | |
| person.identifier.rid | D-4990-2013 | |
| person.identifier.scopus-author-id | 6603741848 | |
| rcaap.rights | closedAccess | por |
| rcaap.type | article | por |
| relation.isAuthorOfPublication | be653ab6-34ec-4329-972a-eee990a7ec66 | |
| relation.isAuthorOfPublication | 09f6a7bd-2f15-42b0-adc5-04bd22210519 | |
| relation.isAuthorOfPublication.latestForDiscovery | be653ab6-34ec-4329-972a-eee990a7ec66 |