Logo do repositório
 
Publicação

Wine astringent compounds monitored by an electrochemical biosensor

2022Artigo científico Acesso aberto
Ver registo simples
dc.contributor.authorCosta, Joana J.
dc.contributor.authorMoreira, Felismina
dc.contributor.authorSoares, Susana
dc.contributor.authorBrandão, Elsa
dc.contributor.authorMateus, Nuno
dc.contributor.authorFreitas, Victor de
dc.contributor.authorSales, Goreti
dc.date.accessioned2023-01-26T09:25:37Z
dc.date.available2023-01-26T09:25:37Z
dc.date.issued2022
dc.description.abstractAn innovative approach for monitoring astringent polyphenols in beverages (wines) is described, consisting of an electrochemical biosensor constructed by adsorbing salivary α-amylase or proline-rich protein (PRP) onto amined gold screen-printed electrodes. Interaction with polyphenols was tested using pentagalloyl glucose (PGG) as a standard, an important representative element for astringency. The analytical properties of the resulting biosensors were evaluated by electrochemical impedance spectroscopy at different pHs. The PRP-biosensor was able to bind to PGG with higher sensitivity, displaying lower limit of the linear range of 0.6 μM. Wine samples were tested to prove the concept and the concentrations obtained ranged from 0.17 to 4.7 μM, as expressed in PGG units. The effects of side-compounds on PRP and on α-amylase binding to PGG were tested (gallic acid, catechin, ethanol, glucose, fructose and glycerol) and considered negligible. Overall, concentrations > 1.0 μM in PGG units are signaling electrochemical impedance, providing a quantitative monitoring of astringent compounds.pt_PT
dc.description.sponsorshipFoodNanoSense, Compreensão do amargor e adstringência dos polifenóis presentes em alimentos para o desenvolvimento de um biossensor, supported by Fundação para a Ciência e Tecnologia, with reference PTDC/AGR-TEC/6547/2014.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.foodchem.2022.133587pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/21876
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0308814622015497?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectPolyphenolspt_PT
dc.subjectProline Rich Proteinpt_PT
dc.subjectα-Amylasept_PT
dc.subjectScreen-printed electrodespt_PT
dc.subjectAstringent compoundspt_PT
dc.subjectElectrochemical biosensorpt_PT
dc.titleWine astringent compounds monitored by an electrochemical biosensorpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FAGR-TEC%2F6547%2F2014/PT
oaire.citation.startPage133587pt_PT
oaire.citation.titleFood Chemistrypt_PT
oaire.citation.volume395pt_PT
oaire.fundingStream9471 - RIDTI
person.familyNameJorge da Costa
person.familyNameMoreira
person.familyNameSoares
person.familyNameFerreira Azevedo Brandão
person.familyNameMateus
person.familyNameFerreira Sales
person.givenNameJoana
person.givenNameFelismina
person.givenNameSusana
person.givenNameElsa Judite
person.givenNameNuno
person.givenNameMaria Goreti
person.identifier1589429
person.identifierL-8759-2014
person.identifierhttps://scholar.google.com/citations?user=OjvsJ_UAAAAJ&hl=pt-PT&oi=ao
person.identifier826970
person.identifier.ciencia-idC917-6A46-A270
person.identifier.ciencia-idC118-BA75-9A73
person.identifier.ciencia-idAA1E-DF51-6EA8
person.identifier.ciencia-idDC1E-7EC3-74FD
person.identifier.ciencia-idE31F-1630-42BB
person.identifier.orcid0000-0002-8654-6287
person.identifier.orcid0000-0003-4237-8952
person.identifier.orcid0000-0002-8313-8181
person.identifier.orcid0000-0003-3461-0635
person.identifier.orcid0000-0002-9318-9732
person.identifier.orcid0000-0001-9936-7336
person.identifier.ridK-9199-2013
person.identifier.scopus-author-id23486193000
person.identifier.scopus-author-id56368171400
person.identifier.scopus-author-id6603373064
person.identifier.scopus-author-id7005174717
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication0be35b08-fad4-4261-b3ba-8bb76d946232
relation.isAuthorOfPublication9c13153d-a73a-49ff-b7f6-c9733bc6725e
relation.isAuthorOfPublication2e8b0b05-8a91-4796-8126-b854337285f6
relation.isAuthorOfPublication9301ee58-2f93-4f09-adba-877d6eebf1b2
relation.isAuthorOfPublicationca5c1e63-982c-4a7a-9571-789c8a419553
relation.isAuthorOfPublicationb6d3a2d0-608a-4a92-834b-8d7b29069fef
relation.isAuthorOfPublication.latestForDiscovery0be35b08-fad4-4261-b3ba-8bb76d946232
relation.isProjectOfPublication67697f8c-65cb-432f-bc27-91f971bc7df1
relation.isProjectOfPublication.latestForDiscovery67697f8c-65cb-432f-bc27-91f971bc7df1

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
ART11_BioMark_2022.pdf
Tamanho:
2.64 MB
Formato:
Adobe Portable Document Format
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

ISEP – BioMark – Artigos