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Ultrasensitive detection of ovarian cancer marker using immunoliposomes and gold nanoelectrodes

dc.contributor.authorViswanathan, Subramanian
dc.contributor.authorRani, Chinnakkaruppanan
dc.contributor.authorDelerue-Matos, Cristina
dc.date.accessioned2013-09-27T14:17:43Z
dc.date.available2013-09-27T14:17:43Z
dc.date.issued2012
dc.description.abstractMucin-16 (MUC16) is the established ovarian cancer marker used to follow the disease during or after treatment for epithelial ovarian cancer. The emerging science of cancer markers also demands for the new sensitive detection methods. In this work, we have developed an electrochemical immunosensor for antigen MUC16 using gold nanoelectrode ensemble (GNEE) and ferrocene carboxylic acid encapsulated liposomes tethered with monoclonal anti-Mucin-16 antibodies ( MUC16). GNEEs were fabricated by electroless deposition of the gold within the pores of polycarbonate track-etched membranes. Afterwards, MUC16 were immobilized on preformed self-assembled monolayer of cysteamine on the GNEE via cross-linking with EDC-Sulfo-NHS. A sandwich immunoassay was performed on MUC16 functionalized GNEE with MUC16 and immunoliposomes. The differential pulse voltammetry was employed to quantify the faradic redox response of ferrocene carboxylic acid released from immunoliposomes. The dose–response curve for MUC16 concentration was found between the range of 0.001–300 U mL−1. The lowest detection limit was found to be 5 × 10−4 U mL−1 (S/N = 3). We evaluated the performance of this developed immunosensor with commercial ELISA assay by comparing results obtained from spiked serum samples and real blood serum samples from volunteers.por
dc.identifier.doi10.1016/j.aca.2012.03.025pt_PT
dc.identifier.issn0003-2670
dc.identifier.urihttp://hdl.handle.net/10400.22/2034
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.ispartofseriesAnalytica Chimica Acta; Vol. 726
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0003267012004424por
dc.subjectImmunosensorpor
dc.subjectOvarian cancerpor
dc.subjectMucin-16por
dc.subjectGold nanoelectrodespor
dc.subjectLiposomepor
dc.titleUltrasensitive detection of ovarian cancer marker using immunoliposomes and gold nanoelectrodespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage84por
oaire.citation.startPage79por
oaire.citation.titleAnalytica Chimica Acta
oaire.citation.volume726por
oaire.citation.volumevol. 726
person.familyNameDelerue-Matos
person.givenNameCristina
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id6603741848
rcaap.rightsclosedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication.latestForDiscovery09f6a7bd-2f15-42b0-adc5-04bd22210519

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