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Ionizing radiation from radiopharmaceuticals and the human gut microbiota: An ex vivo approach

2022-09-16Journal article Open access
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dc.contributor.authorFernandes, Ana
dc.contributor.authorOliveira, Ana
dc.contributor.authorGuedes, Carla
dc.contributor.authorFernandes, Rúben
dc.contributor.authorSoares, Raquel
dc.contributor.authorBarata, Pedro
dc.date.accessioned2023-02-01T15:03:53Z
dc.date.available2023-02-01T15:03:53Z
dc.date.issued2022-09-16
dc.description.abstractThis study aimed to determine the effect of three widely used radiopharmaceuticals with intestinal excretion on selected relevant bacteria that are part of the human gut microbiota, using an ex vivo approach. Fecal samples obtained from healthy volunteers were analyzed. Each sample was divided into four smaller aliquots. One served as the non-irradiated control. The other three were homogenized with three radiopharmaceutical solutions ([131I]NaI, [99mTc]NaTcO4, and [223Ra]RaCl2). Relative quantification of each taxa was determined by the 2−ΔΔC method, using the ribosomal gene 16S as an internal control (primers 534/385). Twelve fecal samples were analysed: three controls and nine irradiated. Our experiment showed fold changes in all analyzed taxa with all radiopharmaceuticals, but results were more significant with I-131, ranging from 1.87–83.58; whereas no relevant differences were found with Tc-99m and Ra-223, ranging from 0.98–1.58 and 0.83–1.97, respectively. This study corroborates limited existing research on how ionizing radiation changes the gut microbiota composition, providing novel data regarding the ex vivo effect of radiopharmaceuticals. Our findings justify the need for future larger scale projects.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFernandes, A., Oliveira, A., Guedes, C., Fernandes, R., Soares, R., & Barata, P. (2022). Ionizing Radiation from Radiopharmaceuticals and the Human Gut Microbiota: An Ex Vivo Approach. International Journal of Molecular Sciences, 23(18), 10809. https://doi.org/10.3390/ijms231810809pt_PT
dc.identifier.doi10.3390/ijms231810809pt_PT
dc.identifier.eissn1422-0067
dc.identifier.urihttp://hdl.handle.net/10400.22/22071
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/23/18/10809pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectRadiation toxicitypt_PT
dc.subjectCommensal microbiotapt_PT
dc.subjectRadiopharmaceuticalspt_PT
dc.subjectRadioiodinept_PT
dc.subjectRadium-223pt_PT
dc.subjectTechnetium-99mpt_PT
dc.subjectIonizing radiationpt_PT
dc.subjectGut microbiotapt_PT
dc.titleIonizing radiation from radiopharmaceuticals and the human gut microbiota: An ex vivo approachpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage13pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume23(18)pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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