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Life and death of Pseudokirchneriella subcapitata: physiological changes during chronological aging

2022Journal article Restricted access
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dc.contributor.authorMachado, Manuela Dias
dc.contributor.authorSoares, Eduardo V.
dc.date.accessioned2023-01-18T11:17:55Z
dc.date.embargo2035
dc.date.issued2022
dc.description.abstractThe green alga Pseudokirchneriella subcapitata is widely used in ecotoxicity assays and has great biotechnological potential as feedstock. This work aims to characterize the physiology of this alga associated with the aging resulting from the incubation of cells for 21 days, in the OECD medium, with continuous agitation and light exposure, in a batch mode. After inoculation, cells grow exponentially during 3 days, and the culture presents a typical green color. In this phase, “young” algal cells present, predominantly, a lunate morphology with the chloroplast occupying a large part of the cell, maximum photosynthetic activity and pigments concentration, and produce starch as a reserve material. Between the 5th and the 12th days of incubation, cells are in the stationary phase. The culture becomes less green, and the cells stop dividing (≥ 99% have one nucleus) and start to age. “Old” algal cells present chloroplast shrinkage, an abrupt decline of chlorophylls content, and photosynthetic capacity (Fv/Fm and ɸPSII), accompanied by a degradation of starch and an increase of neutral lipids content. The onset of the death phase occurs after the 12th day and is characterized by the loss of cell membrane integrity of some algae (cell death). The culture stays, progressively, yellow, and the majority of the population (~93%) is composed of live cells, chronologically “old,” with a significant drop in photosynthetic activity (decay > 75% of Fv/Fm and ɸPSII) and starch content. The information here achieved can be helpful when exploring the potential of this alga in toxicity studies or in biotechnological applications.pt_PT
dc.description.sponsorshipThis study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit. Manuela D. Machado thanks FCT for funding through program DL 57/2016-Norma Transitória.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s00253-022-12267-5pt_PT
dc.identifier.issn0175-7598
dc.identifier.urihttp://hdl.handle.net/10400.22/21634
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationCentre of Biological Engineering of the University of Minho
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s00253-022-12267-5pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectCell-division cyclept_PT
dc.subjectCell deathpt_PT
dc.subjectConditional senescencept_PT
dc.subjectPhotosynthesispt_PT
dc.subjectStationary phasept_PT
dc.subjectReserve material (starch and lipids)pt_PT
dc.titleLife and death of Pseudokirchneriella subcapitata: physiological changes during chronological agingpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre of Biological Engineering of the University of Minho
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT
oaire.citation.endPage8258pt_PT
oaire.citation.issue24pt_PT
oaire.citation.startPage8245pt_PT
oaire.citation.titleApplied Microbiology and Biotechnologypt_PT
oaire.citation.volume106pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameDias Machado
person.familyNameSoares
person.givenNameManuela
person.givenNameEduardo
person.identifier93637
person.identifier.ciencia-id491C-EDE6-F3F3
person.identifier.ciencia-idD612-0D0E-4984
person.identifier.orcid0000-0003-3519-8654
person.identifier.orcid0000-0003-2280-5291
person.identifier.ridO-5908-2015
person.identifier.scopus-author-id23485908300
person.identifier.scopus-author-id7102060173
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication98b9a815-89d6-45cf-8d6d-e9de0599a8cd
relation.isAuthorOfPublicationb3ebdee1-84fa-4183-a1d8-c3cdcaaef219
relation.isAuthorOfPublication.latestForDiscoveryb3ebdee1-84fa-4183-a1d8-c3cdcaaef219
relation.isProjectOfPublication222bb02c-0380-41b1-8083-7c6f585e2db3
relation.isProjectOfPublication.latestForDiscovery222bb02c-0380-41b1-8083-7c6f585e2db3

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