Repository logo
 
Publication

Liquid Redox Probe-Free Plastic Antibody Development for Malaria Biomarker Recognition

datacite.subject.fosEngenharia e Tecnologia
datacite.subject.sdg09:Indústria, Inovação e Infraestruturas
dc.contributor.authorGlória, Juliane Corrêa
dc.contributor.authorOliveira, Daniela S.
dc.contributor.authorGandarilla, Ariamna Dip
dc.contributor.authorBarcelay, Yonny Romaguera
dc.contributor.authorMariúba, Luis André Morais
dc.contributor.authorNogueira, Paulo Afonso
dc.contributor.authorBrito, Walter Ricardo
dc.contributor.authorMoreira, Felismina T. C.
dc.contributor.authorMoreira, Felismina
dc.contributor.authorDOS SANTOS OLIVEIRA, DANIELA
dc.date.accessioned2025-02-11T19:15:00Z
dc.date.available2025-02-11T19:15:00Z
dc.date.issued2024
dc.description.abstractMalaria is a major public health challenge worldwide and requires accurate and efficient diagnostic methods. Traditional diagnostic approaches based on antigen–antibody interactions are associated with ethical and economic concerns. Molecularly imprinted polymers (MIPs) offer a promising alternative by providing a complementary polymer structure capable of selectively binding target molecules. In this study, we developed a liquid, redox-probe-free, MIP-based electrochemical biosensor to detect the Plasmodium falciparum malaria marker histidine-rich protein (HRP2) at the point-of-care (PoC). The imprinting phase consists of the electropolymerization of the monomer methylene blue (MB) in the presence of the target protein HRP2 at the working electrode (WE) of the modified carbon screen printed electrode (C-SPE). Subsequent removal of the protein with proteinase K and oxalic acid yielded the MIP material. The sensor assembly was monitored by cyclic voltammetry (CV), Raman spectroscopy and scanning electron microscopy (SEM). The analytical performance of the biosensor was evaluated by square-wave voltammetry (SWV) using calibration curves in buffer and serum with a detection limit of 0.43 ± 0.026 pg mL–1. Selectivity studies showed minimal interference, indicating a highly selective assay. Overall, our approach to detect the HRP2 infection marker offers simplicity, cost-effectiveness and reliability. In particular, the absence of a redox solution simplifies detection, as the polymer itself is electroactive and exhibits oxidation and reduction peaks.eng
dc.description.sponsorshipThis work was partially supported by the Portuguese Foundation for Science and Technology (FCT), through grants UIDB/04730/2020; UIDP/04730/2020 and FCT - CAPES/FCT – Edital n ° 34/2018.
dc.identifier.citationGlória, J. C., Oliveira, D. S., Gandarilla, A. D., Barcelay, Y. R., Mariúba, L. A. M., Nogueira, P. A., Brito, W. R., & Moreira, F. T. C. (2024). Liquid Redox Probe-Free Plastic Antibody Development for Malaria Biomarker Recognition. ACS Omega, 9(30), 33130-33139. https://doi.org/10.1021/acsomega.4c04543
dc.identifier.doi10.1021/acsomega.4c04543
dc.identifier.issn2470-1343
dc.identifier.urihttp://hdl.handle.net/10400.22/29475
dc.language.isoeng
dc.peerreviewedyes
dc.publisherACS Publications
dc.relationCenter for Innovation in Industrial Engineering and Technology
dc.relationCenter for Innovation in Industrial Engineering and Technology
dc.relation.hasversionhttps://pubs.acs.org/doi/10.1021/acsomega.4c04543
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectElectrodes
dc.subjectParasites
dc.subjectPlastics
dc.subjectPolymers
dc.subjectSensors
dc.titleLiquid Redox Probe-Free Plastic Antibody Development for Malaria Biomarker Recognitioneng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCenter for Innovation in Industrial Engineering and Technology
oaire.awardTitleCenter for Innovation in Industrial Engineering and Technology
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04730%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04730%2F2020/PT
oaire.citation.endPage33139
oaire.citation.issue30
oaire.citation.startPage33130
oaire.citation.titleACS Omega
oaire.citation.volume9
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameMoreira
person.familyNameDOS SANTOS OLIVEIRA
person.givenNameFelismina
person.givenNameDANIELA
person.identifier1589429
person.identifier.ciencia-idC917-6A46-A270
person.identifier.ciencia-idD319-DEC9-9B9F
person.identifier.orcid0000-0003-4237-8952
person.identifier.orcid0000-0002-6493-0237
person.identifier.orcid0000-0003-4237-8952
person.identifier.orcid0000-0001-7159-2638
person.identifier.scopus-author-id23486193000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isAuthorOfPublication9c13153d-a73a-49ff-b7f6-c9733bc6725e
relation.isAuthorOfPublication1b256c49-05f4-4958-9a13-8cc1b795412b
relation.isAuthorOfPublication.latestForDiscovery9c13153d-a73a-49ff-b7f6-c9733bc6725e
relation.isProjectOfPublicationcdbfce2f-6ff0-4d59-a7c6-96c99d52a570
relation.isProjectOfPublicationa21a18a5-dd47-49e2-8af6-3e4c8cfaaeea
relation.isProjectOfPublication.latestForDiscoverycdbfce2f-6ff0-4d59-a7c6-96c99d52a570

Files

Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
ART_JCGlória_CIET-LabRise_2024_10.pdf
Size:
4.48 MB
Format:
Adobe Portable Document Format
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
4.03 KB
Format:
Item-specific license agreed upon to submission
Description: