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Untangling sex differences in glia-to-neuron communication in chronic alcohol exposure

2025-05Conference poster Open access
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dc.contributor.authorPacheco, Raquel
dc.contributor.authorCanedo, Teresa
dc.contributor.authorRodrigues, Ana Margarida
dc.contributor.authorMoreira, Joana
dc.contributor.authorRelvas, João B.
dc.contributor.authorVieira, Cristina P.
dc.contributor.authorSocodato, Renato
dc.contributor.authorSumavielle, Teresa
dc.contributor.authorSummavielle, Teresa
dc.date.accessioned2025-11-12T12:43:55Z
dc.date.available2025-11-12T12:43:55Z
dc.date.issued2025-05
dc.description.abstractExcessive alcohol consumption is global health challenge with profound neurobiological consequences. Our laboratory has shown that alcohol exposure induces reactive astrocytic changes, affecting gene expression, activity, and proliferation, while also altering microglial morphology and immune responses [1]. This study aims to characterise sex-specific effects of chronic alcohol consumption on the prefrontal cortex (PFC), focusing on glial cell morphology, synaptic density, and behaviour. Adult mice underwent a voluntary drinking model using an intermittent ‘every-other-day’ (EOD) paradigm with 15% (v/v) ethanol for three weeks. Behavioural tests for anxiety, depression, and memory were conducted before sacrifice. Brains were processed for glial cell analysis using immunohistochemistry and confocal microscopy. Synaptosomes were isolated for proteomics and analysis used STRING and Panther. Preliminary data reveal sex-dependent glial and synaptic adaptations. Males showed increased astrocyte volume in the ventromedial PFC (vmPFC) and hyper-ramification in the ventrolateral PFC (vlPFC), while females showed reductions in astrocyte size and complexity. Microglia also displayed sex-specific changes: males had decreased microglial volume in the vlPFC, while females exhibited increased microglial size. Inhibitory synapse density was elevated in males, while females showed an increase in excitatory synapses. These changes correlated with behavioural differences—males displayed heightened anxiety, whereas females exhibited reduced anxiety. Proteomic analysis further supports sex-dependent molecular adaptations: male-enriched proteins were linked to myelination and glial development, while female-specific proteins were associated with mRNA processing, RNA transport, and axonogenesis. Chronic alcohol exposure induces sex-specific neuroimmune and synaptic alterations, potentially contributing to differential susceptibility to alcohol use disorders (AUD).por
dc.identifier.citationPacheco, R., Canedo, T., Rodrigues, A. M., Moreira, J., Relvas, J. B., Vieira, C. P., Socodato, R., & Sumavielle, T. (2025). Untangling sex differences in glia-to-neuron communication in chronic alcohol exposure. Livro de Resumos do 18º Encontro de Investigação Jovem da U.Porto, 999–1000. https://www.up.pt/ijup/wp-content/uploads/sites/892/2025/06/Livro-de-Resumos_IJUP-2025.pdf
dc.identifier.isbn978-989-746-418-8
dc.identifier.urihttp://hdl.handle.net/10400.22/30848
dc.language.isoeng
dc.peerreviewedyes
dc.publisherUniversidade do Porto
dc.relationPTDC/SAU-TOX/0067/2021
dc.relation.hasversionhttps://www.up.pt/ijup/wp-content/uploads/sites/892/2025/06/Livro-de-Resumos_IJUP-2025.pdf
dc.rights.uriN/A
dc.subjectNeuroimmune response
dc.subjectGlial activation
dc.subjectAlcohol-related neuroplasticity
dc.subjectPFC Dysfunction
dc.subjectSex differences
dc.titleUntangling sex differences in glia-to-neuron communication in chronic alcohol exposurepor
dc.typeconference poster
dspace.entity.typePublication
oaire.citation.conferenceDate2025-05
oaire.citation.conferencePlacePorto
oaire.citation.endPage1000
oaire.citation.startPage999
oaire.citation.titleLivro de Resumos do 18º Encontro de Investigação Jovem da U.Porto
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameSummavielle
person.givenNameTeresa
person.identifier677706
person.identifier.ciencia-idC41E-0816-5C85
person.identifier.orcid0000-0003-2548-6281
person.identifier.ridC-9776-2012
person.identifier.scopus-author-id6603092949
relation.isAuthorOfPublication207ee2de-85a0-4144-9e7e-b376c600e065
relation.isAuthorOfPublication.latestForDiscovery207ee2de-85a0-4144-9e7e-b376c600e065

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