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Plastic antibody for the electrochemical detection of bacterial surfaceproteins

2016Journal article Open access
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dc.contributor.authorKhan, M. Azizur R.
dc.contributor.authorMoreira, Felismina T. C.
dc.contributor.authorRiu, Jordi
dc.contributor.authorFerreira Sales, Maria Goreti
dc.date.accessioned2017-06-29T10:59:51Z
dc.date.available2017-06-29T10:59:51Z
dc.date.issued2016
dc.description.abstractThis work presents a novel molecularly imprinted polymer (MIP) for the indirect detection of bacteria, by targeting an outer membrane protein on a disposable device. Protein A (PA) was selected for this purpose, as a representative protein of the outer surface of Staphylococcus aureus. The imprinted polymer was assembled directly on a film of single walled carbon nanotubes (SWCNTs), placed on screen-printed electrodes (SPEs). The MIP material was produced by electropolymerizing 3-aminophenol in the presence of the protein template (PA) using cyclic voltammetry (CV). The proteins entrapped at the polymeric backbone were digested by the action of proteolytic activity of proteinase K and then washed away to create vacant sites. The performance of the corresponding imprinted and non-imprinted electrodes was evaluated by EIS and the effect of several variables, such as monomer and template concentrations, or thickness of imprint-ing surface, was controlled and optimized by the number of CV cycles. The detection limit of the MIP-based sensors was 0.60 nM in MES buffer. High repeatability and good selectivity were observed in the presence of a model protein BSA. The sensor performance was also tested to check the effect of inorganic ions in tap water. The detection limit observed was 16.83 nM, with a recovery factor of 91.1 ± 6.6%. The sensor described in this work is a potential tool for screening PA on-site, due to the simplicity of fabrication, disposability, short response time, low cost, good sensitivity and selectivity.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.snb.2016.04.075pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/9967
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0925400516305573pt_PT
dc.subjectPlastic antibodypt_PT
dc.subjectMolecularly imprinted polymerpt_PT
dc.subjectElectropolymerizationpt_PT
dc.subjectBacterial surface proteinspt_PT
dc.subjectStaphylococcus aureuspt_PT
dc.subjectScreen-printed electrodespt_PT
dc.subjectDisposable devicept_PT
dc.titlePlastic antibody for the electrochemical detection of bacterial surfaceproteinspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleSensors and Actuators B: Chemicalpt_PT
person.familyNameMoreira
person.familyNameFerreira Sales
person.givenNameFelismina
person.givenNameMaria Goreti
person.identifier1589429
person.identifier826970
person.identifier.ciencia-idC917-6A46-A270
person.identifier.ciencia-idE31F-1630-42BB
person.identifier.orcid0000-0003-4237-8952
person.identifier.orcid0000-0001-9936-7336
person.identifier.ridK-9199-2013
person.identifier.scopus-author-id23486193000
person.identifier.scopus-author-id7005174717
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication9c13153d-a73a-49ff-b7f6-c9733bc6725e
relation.isAuthorOfPublicationb6d3a2d0-608a-4a92-834b-8d7b29069fef
relation.isAuthorOfPublication.latestForDiscoveryb6d3a2d0-608a-4a92-834b-8d7b29069fef

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