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Biosensor for direct bioelectrocatalysis detection of nitric oxide using nitric oxide reductase incorporated in carboxylated single-walled carbon nanotubes / lipidic bilayer nanocomposite

2019-06-01Journal article Restricted access
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dc.contributor.authorGomes, F.O.
dc.contributor.authorMaia, L.B.
dc.contributor.authorLoureiro, J.A.
dc.contributor.authorPereira, M.C.
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorMoura, I.
dc.contributor.authorMoura, J.J.G.
dc.contributor.authorMorais, Simone
dc.date.accessioned2019-10-07T10:43:23Z
dc.date.embargo2119
dc.date.issued2019-06-01
dc.description.abstractAn enzymatic biosensor based on nitric oxide reductase (NOR; purified from Marinobacter hydrocarbonoclasticus) was developed for nitric oxide (NO) detection. The biosensor was prepared by deposition onto a pyrolytic graphite electrode (PGE) of a nanocomposite constituted by carboxylated single-walled carbon nanotubes (SWCNTs), a lipidic bilayer [1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine (DOPE), 1,2-di-(9Zoctadecenoyl)-3-trimethylammonium-propane (DOTAP), 1,2-distearoyl-sn-glycero-3-phosphoethanolaminepolyethylene glycol (DSPE-PEG)] and NOR. NOR direct electron transfer and NO bioelectrocatalysis were characterized by several electrochemical techniques. The biosensor development was also followed by scanning electron microscopy and Fourier transform infrared spectroscopy. Improved enzyme stability and electron transfer (1.96 × 10−4 cm.s−1 apparent rate constant) was obtained with the optimum SWCNTs/(DOPE:DOTAP:DSPEPEG)/NOR) ratio of 4/2.5/4 (v/v/v), which biomimicked the NOR environment. The PGE/[SWCNTs/(DOPE:DOTAP:DSPE-PEG)/NOR] biosensor exhibited a low Michaelis-Menten constant (4.3 μM), wide linear range (0.44–9.09 μM), low detection limit (0.13 μM), high repeatability (4.1% RSD), reproducibility (7.0% RSD), and stability (ca. 5weeks). Selectivity tests towards L-arginine, ascorbic acid, sodiumnitrate, sodiumnitrite and glucose showed that these compounds did not significantly interfere in NO biosensing (91.0 ± 9.3%–98.4 ± 5.3% recoveries). The proposed biosensor, by incorporating the benefits of biomimetic features of the phospholipid bilayer with SWCNT's inherent properties and NOR bioelectrocatalytic activity and selectivity, is a promising tool for NO.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.bioelechem.2019.01.010
dc.identifier.urihttp://hdl.handle.net/10400.22/14701
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationPTDC/ASP-PES/29547/2017 - CECs(Bio)Sensing - (Bio)sensores para avaliação de contaminantes emergentes em produtos da pescapt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/abs/pii/S1567539418304729?via%3Dihubpt_PT
dc.subjectDirect electron transferpt_PT
dc.subjectEnzymatic biosensorpt_PT
dc.subjectNitric oxide reductasept_PT
dc.titleBiosensor for direct bioelectrocatalysis detection of nitric oxide using nitric oxide reductase incorporated in carboxylated single-walled carbon nanotubes / lipidic bilayer nanocompositept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage86pt_PT
oaire.citation.startPage76pt_PT
oaire.citation.titleBioelectrochemistrypt_PT
oaire.citation.volume127pt_PT
person.familyNameDelerue-Matos
person.familyNameMorais
person.givenNameCristina
person.givenNameSimone
person.identifier1598822
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.ciencia-idB111-BBFB-F200
person.identifier.orcid0000-0002-3924-776X
person.identifier.orcid0000-0001-6433-5801
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id6603741848
person.identifier.scopus-author-id7007053747
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication7a9c7d63-a6c7-4159-b12d-b049b8c5c8f9
relation.isAuthorOfPublication.latestForDiscovery09f6a7bd-2f15-42b0-adc5-04bd22210519

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