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Simple laccase-based biosensor for formetanate hydrochloride quantification in fruits

2014-02Journal article Restricted access
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dc.contributor.authorRibeiro, Francisco Wirley Paulino
dc.contributor.authorBarroso, M. Fátima
dc.contributor.authorMorais, Simone
dc.contributor.authorViswanathan, Subramanian
dc.contributor.authorLima-Neto, Pedro de
dc.contributor.authorCorreia, Adriana N.
dc.contributor.authorOliveira, M. Beatriz P. P.
dc.contributor.authorDelerue-Matos, Cristina
dc.date.accessioned2014-12-22T14:55:48Z
dc.date.available2014-12-22T14:55:48Z
dc.date.issued2014-02
dc.description.abstractThis work describes the development of an electrochemical enzymatic biosensor for quantification of the pesticide formetanate hydrochloride (FMT). It is based on a gold electrode modified with electrodeposited gold nanoparticles and laccase. The principle behind its development relies on FMT's capacity to inhibit the laccase catalytic reaction that occurs in the presence of phenolic substrates. The optimum values for the relevant experimental variables such as gold nanoparticles electrochemical deposition (at − 0.2 V for 100 s), laccase immobilization (via glutaraldehyde cross-linking), laccase concentration (12.4 mg/mL), substrate selection and concentration (5.83×10−5 M of aminophenol), pH (5.0), buffer (Britton–Robinson), and square-wave voltammetric parameters were determined. The developed biosensor was successfully applied to FMT determination in mango and grapes. The attained limit of detection was 9.5×10−8 ± 9.5×10−10 M (0.02 ± 2.6×10−4 mg/kg on a fresh fruit weight basis). Recoveries for the five tested spiking levels ranged from 95.5 ± 2.9 (grapes) to 108.6 ± 2.5% (mango). The results indicated that the proposed device presents suitable characteristics in terms of sensitivity (20.58 ± 0.49 A/μM), linearity (9.43×10−7 to 1.13×10−5 M), accuracy, repeatability (RSD of 1.4%), reproducibility (RSD of 1.8%) and stability (19 days) for testing of compliance with established maximum residue limits of FMT in fruits and vegetables.por
dc.identifier.doi10.1016/j.bioelechem.2013.09.005
dc.identifier.urihttp://hdl.handle.net/10400.22/5272
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.ispartofseriesBioelectrochemistry;Vol. 95
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S1567539413000868por
dc.subjectEnzymatic biosensorpor
dc.subjectGold nanoparticlespor
dc.subjectLaccasepor
dc.subjectFormetanate hydrochloridepor
dc.subjectFruitspor
dc.titleSimple laccase-based biosensor for formetanate hydrochloride quantification in fruitspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage14por
oaire.citation.startPage7por
oaire.citation.titleBioelectrochemistrypor
oaire.citation.volume95por
person.familyNamede Sá Barroso
person.familyNameMorais
person.familyNameDelerue-Matos
person.givenNameMaria de Fátima
person.givenNameSimone
person.givenNameCristina
person.identifier1598822
person.identifier.ciencia-idAF16-22E5-5EBD
person.identifier.ciencia-idB111-BBFB-F200
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0001-9011-5992
person.identifier.orcid0000-0001-6433-5801
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id25631648000
person.identifier.scopus-author-id7007053747
person.identifier.scopus-author-id6603741848
rcaap.rightsclosedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication3d701c85-4580-4614-be6d-5a1abc8cf729
relation.isAuthorOfPublication7a9c7d63-a6c7-4159-b12d-b049b8c5c8f9
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication.latestForDiscovery7a9c7d63-a6c7-4159-b12d-b049b8c5c8f9

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