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Brominated flame retardants effect in MCF-7 cells: Impact on vitamin D pathway

2022-05Journal article Restricted access
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dc.contributor.authorSousa, Sara
dc.contributor.authorMaia, Maria Luz
dc.contributor.authorPestana, Diogo
dc.contributor.authorTeixeira, Diana
dc.contributor.authorÂngelo-Dias, Miguel
dc.contributor.authorMartins, Catarina
dc.contributor.authorBorrego, Luís Miguel
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorCalhau, Conceição
dc.contributor.authorDomingues, Valentina
dc.contributor.authorFaria, Ana
dc.date.accessioned2023-01-30T11:00:43Z
dc.date.embargo2035
dc.date.issued2022-05
dc.description.abstractBrominated flame retardants (BFRs) are persistent environmental pollutants, allowing a constant human exposure which carries several health risks, including the occurrence of breast cancer and vitamin D deficiency. Vitamin D inhibits cell growth and is negatively associated with breast cancer risk. The effect of BFRs in breast cancer and vitamin D pathway is still poorly understood. MCF-7 cells were treated with hexabromocyclododecane (HBCD), 2-ethylhexyl-2,3,4,5-tetrabromobenzoate (TBB), hexabromobenzene (HBB) and pentabromotoluene (PBT) using short and long-term exposure protocols. Viability, proliferation, migration, cell cycle and gene expression were assessed. Gene expression of hVDBP and hCYP2R1 was also evaluated in hepatocytes. Long-term exposure of MCF-7 cells to HBB increased cell proliferation and migration, consequently increasing MMP-9 expression. The vitamin D pathway was also altered by BFRs: cells appeared less prepared to activate and transport vitamin D and the signaling, action and inactivation mechanisms were diminished in the presence of BFRs. Untreated MCF-7 cells showed cell cycle arrest in phase G0/G1 in the presence of activated vitamin D. However, when MCF-7 cells were exposed to BFRs, cell cycle was arrested in phase G2/M, possibly due to DNA damage. Nonetheless, calcitriol seems to be able to mitigate the effect of some BFRs exposure, e.g. PBTpt_PT
dc.description.sponsorshipSara Sousa and Maria Luz Maia are grateful to FCT and ESF (European Social Fund) through POCH (Programa Operacional Capital Humano) for the Ph.D. grants (SFRH/BD/137516/2018 and SFRH/BD/128817/2017, respectively). The work was supported by UIDB/50006/2020 and UIDP/50006/2020, CHRC (UIDB/04923/2020 and UIDP/04923/2020) with funding from FCT/MCTES through PT national funds.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.jsbmb.2022.106079pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/21986
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relationalterado para: “Biomonitoring of lipophilic endocrine disruptors in women: cancer biology and Vitamin D pathways” Vitamin D and Endocrine Disruptors relationship in Breast Cancer Women
dc.relationPortuguese shrimps: nutritional added value and contaminants evaluation. Risk-benefit.
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationComprehensive Health Research Center - Research, Education, Training and Innovation in Clinical research and Public Health
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationComprehensive Health Research Center - Research, Education, Training and Innovation in Clinical research and Public Health
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0960076022000309?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectHexabromocyclododecanept_PT
dc.subjectPentabromotoluenept_PT
dc.subject2-ethylhexyl-2,3,4,5-tetrabromobenzoatept_PT
dc.subjectHexabromobenzenept_PT
dc.subjectBreast cancerpt_PT
dc.subjectCalcitriolpt_PT
dc.titleBrominated flame retardants effect in MCF-7 cells: Impact on vitamin D pathwaypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlealterado para: “Biomonitoring of lipophilic endocrine disruptors in women: cancer biology and Vitamin D pathways” Vitamin D and Endocrine Disruptors relationship in Breast Cancer Women
oaire.awardTitlePortuguese shrimps: nutritional added value and contaminants evaluation. Risk-benefit.
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardTitleComprehensive Health Research Center - Research, Education, Training and Innovation in Clinical research and Public Health
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardTitleComprehensive Health Research Center - Research, Education, Training and Innovation in Clinical research and Public Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F137516%2F2018/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F128817%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04923%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50006%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04923%2F2020/PT
oaire.citation.startPage106079pt_PT
oaire.citation.titleThe Journal of Steroid Biochemistry and Molecular Biologypt_PT
oaire.citation.volume219pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameSousa
person.familyNameGalante Maia
person.familyNameDelerue-Matos
person.familyNameDomingues
person.givenNameSara
person.givenNameMaria da Luz
person.givenNameCristina
person.givenNameValentina Maria Fernandes
person.identifier.ciencia-id8513-3537-E319
person.identifier.ciencia-id161F-62CC-7A37
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.ciencia-id4E16-791D-6664
person.identifier.orcid0000-0002-8291-1160
person.identifier.orcid0000-0003-4383-3822
person.identifier.orcid0000-0002-3924-776X
person.identifier.orcid0000-0003-3472-849X
person.identifier.ridQ-5273-2018
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id57194554670
person.identifier.scopus-author-id56436939000
person.identifier.scopus-author-id6603741848
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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