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Abstract(s)
Environmental pollution continues to be an emerging study field, as there are thousands of
anthropogenic compounds mixed in the environment whose possible mechanisms of toxicity and
physiological outcomes are of great concern. Developing methods to access and prioritize the screening
of these compounds at trace levels in order to support regulatory efforts is, therefore, very important. A
methodology based on solid phase extraction followed by derivatization and gas chromatography-mass
spectrometry analysis was developed for the assessment of four endocrine disrupting compounds
(EDCs) in water matrices: bisphenol A, estrone, 17b-estradiol and 17a-ethinylestradiol. The study was
performed, simultaneously, by two different laboratories in order to evaluate the robustness of the
method and to increase the quality control over its application in routine analysis. Validation was done
according to the International Conference on Harmonisation recommendations and other
international guidelines with specifications for the GC-MS methodology. Matrix-induced
chromatographic response enhancement was avoided by using matrix-standard calibration solutions
and heteroscedasticity has been overtaken by a weighted least squares linear regression model
application. Consistent evaluation of key analytical parameters such as extraction efficiency,
sensitivity, specificity, linearity, limits of detection and quantification, precision, accuracy and
robustness was done in accordance with standards established for acceptance. Finally, the application
of the optimized method in the assessment of the selected analytes in environmental samples suggested
that it is an expedite methodology for routine analysis of EDC residues in water matrices.
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Publisher
Royal Society of Chemistry