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Exploring the anti-cancer properties of pomegranate peel aqueous extract

2023-10-27Journal article Open access
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dc.contributor.authorLuís, Carla
dc.contributor.authorSousa, André P.
dc.contributor.authorCosta, Raquel
dc.contributor.authorMaduro, Ana T.
dc.contributor.authorPais, Patrick J.
dc.contributor.authorSá, Sara
dc.contributor.authorGestoso, Álvaro
dc.contributor.authorFernandes, Flávia
dc.contributor.authorJerónimo, Eliana
dc.contributor.authorSoares, Raquel
dc.contributor.authorFernandes, Rúben
dc.contributor.authorBaylina, Pilar
dc.contributor.authorDuarte, Maria F.
dc.date.accessioned2024-04-09T15:46:05Z
dc.date.available2024-04-09T15:46:05Z
dc.date.issued2023-10-27
dc.description.abstractThe objective of this work is to evaluate the influence of pomegranate peel extract (PPE) in the behavior of breast cell lines (epithelial and tumor type) and related oxidative metabolism. Fruitbased functional foods have been the target of increasing scientific research for their physiological and pathophysiological properties. Pomegranate (Punica granatum) is a suitable example with both prophylactic and medicinal effects. MCF-7 cell line from tumor breast carcinoma, and MCF-10A cell line from normal epithelial mammary gland were used and subjected to different concentrations of PPE, ranging from 1 to 5 mM of gallic acid equivalents (GAE). Viability, proliferation, mobility, and cytotoxicity assays were performed along with the quantification of antioxidant enzymes, namely, catalase, superoxide dismutase (SOD) and reduced (GSH) and oxidized (GSSG) glutathione. We observed a decrease in viability and proliferation of MCF-7 cells, at higher concentrations of PPE, with no influence in epithelial cells. Interestingly, in a concentration-dependent manner, PPE triggered a significant decrease in migration on both cell lines, with a more pronounced effect in breast cancer cell line. Regarding antioxidant enzyme activity, on tumor cells higher concentrations of PPE decreased catalase activity and significantly increased SOD activity. Regarding GSH and GSSG, we observed different expression levels between MCF-7 and MCF-10A, with MCF-7 presenting lower levels compared to MCF-10A. GSH/GSSG ratio was notably higher in MCF-7 at 5 mM GAE. PPE exhibits anti-tumor effects without significantly affecting normal epithelial cells. Our work strengthens the potential antitumoral effect of PPE by reducing MCF-7 cell viability and proliferation through the imbalance of antioxidant enzymes.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLuís, C., Sousa, A. P., Costa, R., Maduro, A. T., Pais, P. J., Sá, S., Gestoso, Á., Fernandes, F., Jerónimo, E., Soares, R., Fernandes, R., Baylina, P., & Duarte, M. F. (2023). Exploring the anti-cancer properties of pomegranate peel aqueous extract. Applied Sciences, 13(21), Artigo 21. https://doi.org/10.3390/app132111773pt_PT
dc.identifier.doi10.3390/app132111773pt_PT
dc.identifier.eissn2076-3417
dc.identifier.urihttp://hdl.handle.net/10400.22/25321
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationFundação para a Ciência e Tecnologia (FCT): NORTE-01-0145-FEDER-000012, UID/BIM/04293/2013. P.B.; FEDER/02/SAICT/2020/072560; FCT UIDB/05183/2020; LA/P/0121/2020;SFRH/BD/146489/2019 (FCT);2022.12441.BD (FCT); 2021.09498.BD (FCT).pt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2076-3417/13/21/11773pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBreast cancerpt_PT
dc.subjectPomegranate peel extractpt_PT
dc.subjectPolyphenolspt_PT
dc.subjectOxidative metabolismpt_PT
dc.subjectOxidative stresspt_PT
dc.subjectAntioxidant activitypt_PT
dc.titleExploring the anti-cancer properties of pomegranate peel aqueous extractpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage11pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleApplied Sciencespt_PT
oaire.citation.volume13(21)pt_PT
person.familyNameBAYLINA MACHADO
person.givenNamePILAR
person.identifier.ciencia-id1419-F23D-4920
person.identifier.orcid0000-0002-3740-862X
person.identifier.ridB-5134-2010
person.identifier.scopus-author-id56534079700
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb1482a24-d9d8-419d-af68-6df1a75afb3f
relation.isAuthorOfPublication.latestForDiscoveryb1482a24-d9d8-419d-af68-6df1a75afb3f

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