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Characterization of antibiotic resistance in enterobacteriaceae from agricultural manure and soil in Portugal

2018-03-08Journal article Restricted access
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dc.contributor.authorAmador, Paula
dc.contributor.authorDuarte, Isabel M.
dc.contributor.authorCosta, R.P. Roberto da
dc.contributor.authorFernandes, Rúben
dc.contributor.authorPrudêncio, Cristina
dc.date.accessioned2023-10-25T13:18:00Z
dc.date.available2023-10-25T13:18:00Z
dc.date.issued2018-03-08
dc.description.abstractIntensive livestock production uses antibiotics to treat and prevent infectious diseases. Antibiotics are expelled unaltered in animal manure, which is often used as a fertilizer. This practice spreads antibiotic residues and antibiotic-resistant bacteria and their genes into the soil. These resistance determinants affect soil microbial communities, raising concerns about the potential environmental and public health risks. This study aims to characterize soil contaminated with antibiotic resistant bacteria and the putative role played by manure as a vector for these resistance determinants. Six poultry and dairy cattle farms in the central region of Portugal were sampled. Ampicillin-resistant Enterobacteriaceae isolates from manure and manured-soil samples were tested for their resistance profile to 13 antibiotics and the presence of tetracycline and sulfonamide resistance genes. High multidrug resistance rates (>70%) were observed in both manure and soil samples. This resistance was higher in the poultry samples; manured-soil isolates were more resistant to cefoxitin (91.7%), sulfamethoxazole/trimethoprim (79.2%), chloramphenicol (79.2%), and to a lesser extent tetracycline (12.5%). Manure isolates have high resistance frequency to tetracycline (73.9%), amoxicillin with clavulanic acid (66.0%), chloramphenicol (65.3%), and sulfamethoxazole with trimethoprim (36.5%). Manure isolates revealed high frequency of tet(A), sul1, and sul3 genes. The multiple antibiotic resistance index reveals a putative risk of soil contamination due to antibiotic exposure. The results obtained are important for soil management regarding resistance determinants spread through agricultural practices. To our knowledge, this is the first study of tetracycline and sulfonamide resistance in Portuguese manure and soil.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAmador, P., Duarte, I. M., Roberto da Costa, R. P., Fernandes, R., & Prudêncio, C. (2018). Characterization of Antibiotic Resistance in Enterobacteriaceae From Agricultural Manure and Soil in Portugal. Soil Science, 182(8), 292. https://doi.org/10.1097/SS.0000000000000222pt_PT
dc.identifier.doi10.1097/SS.0000000000000222pt_PT
dc.identifier.isbn1538-9243
dc.identifier.issn0038-075X
dc.identifier.urihttp://hdl.handle.net/10400.22/23775
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWolters Kluwer Healthpt_PT
dc.relationThis work is funded by National Funds through the FCT (Portuguese Foundation for Science and Technology) under project UID/ AMB/00681/2013pt_PT
dc.relation.publisherversionhttps://journals.lww.com/soilsci/abstract/2017/08000/characterization_of_antibiotic_resistance_in.4.aspxpt_PT
dc.subjectAntibiotic-resistant bacteriapt_PT
dc.subjectEmergent contaminantspt_PT
dc.subjectLivestock manurept_PT
dc.subjectManured soilpt_PT
dc.subjectMultidrug resistancept_PT
dc.titleCharacterization of antibiotic resistance in enterobacteriaceae from agricultural manure and soil in Portugalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage301pt_PT
oaire.citation.startPage292pt_PT
oaire.citation.titleSoil Sciencept_PT
oaire.citation.volume182 (8)pt_PT
person.familyNameFernandes
person.familyNamePrudêncio
person.givenNameRúben
person.givenNameCristina
person.identifier635792
person.identifier1200571
person.identifier.ciencia-id0D1F-4090-E82A
person.identifier.ciencia-idC81E-F4EE-FADE
person.identifier.orcid0000-0001-8933-3984
person.identifier.orcid0000-0002-9920-936X
person.identifier.scopus-author-id57640135700
person.identifier.scopus-author-id6508057930
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication9e6c397c-62d8-4a40-8ec7-ad05ae0ebcc4
relation.isAuthorOfPublication881a8ad5-ab13-4e49-89f4-08ca61cc81e3
relation.isAuthorOfPublication.latestForDiscovery9e6c397c-62d8-4a40-8ec7-ad05ae0ebcc4

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